A kind of waxy corn pollen induction medium formula
A technology for inducing medium and waxy corn, applied in the field of agricultural science, can solve the problems of low efficiency of corn flower cultivation, low efficiency of corn pollen cultivation and breeding, and difficulty in obtaining selective populations, etc.
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Embodiment 1
[0013] Prepare the culture medium with the following formula:
[0014] KNO 3 2650mg / L, (NH 4 ) 2 SO 4 350mg / L, KH 2 PO 4 260mg / L, CaCl 2 ?2H 2 O150mg / L, MgSO 4 ?7H 2 O220mg / L, Na 2 -EDTA37.5mg / L, FeSO 4 ?7H 2 O28mg / L, MnSO 4 ?4H 2 O7mg / L, ZnSO 4 ?7H 2 O1.8mg / L, H 3 BO 3 2.0mg / L, KI1.2mg / L, Na 2 MoO 4 ?2H 2 O0.175mg / L, CoCl 2 ?6H 2 O0.0175mg / L, CuSO 4 ?5H 2 O0.0175mg / L, glycerin 3mg / L, glycine 1.0mg / L, folic acid 0.3mg / L, alanine 1.5mg / L, lysine 1.7mg / L, vitamin B11.0mg / L, vitamin B61 .0mg / L, niacin 0.8mg / L, sucrose 25g / L, glucose 15g / L, plant gel 4.0g / L;
[0015] 2,4-D1.7mg / L, KT0.8mg / L, complex nucleic acid 0.3mg / L, L-proline 80mg / L, hydrolyzed protein 0.6g / L, colchicine 0.15mg / L, biotin 0.1mg / L, activated carbon 2.75g / L, pH5.9.
[0016] The waxy maize varieties Shennongnuo and Suyunuo 2 were selected as pollen culture donors, and single-core maize tassels in the middle and late stages were collected, stored in a dark incubator at 6°C for low-temper...
Embodiment 2
[0018] Prepare the medium with the following formula (preferred concentration of alanine added):
[0019] KNO 3 2650mg / L, (NH 4 ) 2 SO 4 350mg / L, KH 2 PO 4 260mg / L, CaCl 2 ?2H 2 O150mg / L, MgSO 4 ?7H 2 O220mg / L, Na 2 -EDTA37.5mg / L, FeSO 4 ?7H 2 O28mg / L, MnSO 4 ?4H 2 O7mg / L, ZnSO 4 ?7H 2 O1.8mg / L, H 3 BO 3 2.0mg / L, KI1.2mg / L, Na 2 MoO 4 ?2H 2 O0.175mg / L, CoCl 2 ?6H 2 O0.0175mg / L, CuSO 4 ?5H 2O0.0175mg / L, glycerin 3mg / L, glycine 1.0mg / L, folic acid 0.3mg / L, lysine 1.7mg / L, vitamin B11.0mg / L, vitamin B61.0mg / L, niacin 0.8 mg / L, sucrose 25g / L, glucose 15g / L, plant gel 4.0g / L;
[0020] 2,4-D1.7mg / L, KT0.8mg / L, complex nucleic acid 0.3mg / L, L-proline 80mg / L, hydrolyzed protein 0.6g / L, colchicine 0.15mg / L, biotin 0.1mg / L, activated carbon 2.75g / L, pH5.9.
[0021] The added concentration of alanine is set in the following 8 types: 0; 0.5mg / L; 1mg / L; 2mg / L; 2.5mg / L; 3mg / L; 3.5mg / L; 4mg / L.
[0022] The two waxy corn varieties were also selected as pollen donor...
Embodiment 3
[0024] Prepare the medium with the following formula (optimization of carbon source combination and added concentration):
[0025] KNO 3 2650mg / L, (NH 4 ) 2 SO 4 350mg / L, KH 2 PO 4 260mg / L, CaCl 2 ?2H 2 O150mg / L, MgSO 4 ?7H 2 O220mg / L, Na 2 -EDTA37.5mg / L, FeSO 4 ?7H 2 O28mg / L, MnSO 4 ?4H 2 O7mg / L, ZnSO 4 ?7H 2 O1.8mg / L, H 3 BO 3 2.0mg / L, KI1.2mg / L, Na 2 MoO 4 ?2H 2 O0.175mg / L, CoCl 2 ?6H 2 O0.0175mg / L, CuSO 4 ?5H 2 O0.0175mg / L, glycerin 3mg / L, glycine 1.0mg / L, folic acid 0.3mg / L, alanine 1.5mg / L, lysine 1.7mg / L, vitamin B11.0mg / L, vitamin B61 .0mg / L, niacin 0.8mg / L, vegetable gel 4.0g / L;
[0026] 2,4-D1.7mg / L, KT0.8mg / L, complex nucleic acid 0.3mg / L, L-proline 80mg / L, hydrolyzed protein 0.6g / L, colchicine 0.15mg / L, biotin 0.1mg / L, activated carbon 2.75g / L, pH5.9.
[0027] The combination of carbon sources and the added concentration are set to the following 8 types: sucrose 40g / L; sucrose 35g / L, glucose 5g / L; sucrose 30g / L, glucose 10g / L; sucrose 20g...
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