Modulation Method of Biological Parameters of Influent Water for Ship Ballast Water Land-Based Test
A land-based test and biological parameter technology, applied in the field of marine engineering equipment, can solve problems such as the lack of modulation methods, achieve the effects of prolonging the testable time, easily obtaining materials, and solving the modulation problem of biological parameters
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Embodiment 1
[0025] Qingdao, summer, detection of organisms >50 µm in 20L natural seawater, meeting the requirements of three categories and five categories, concentration 20ind / m 3 , only Chlorophyta and Diatoms were found at 10-50 µm in 1L seawater, which did not meet the requirements of three categories and five categories, the concentration was 15ind / mL, and the number of heterotrophic bacteria was 10 2 cfu / mL.
[0026] Using the three-stage cultivation method, Sclerophyllum qingdao was cultivated at a temperature of 20°C with ventilation. After 12 days of cultivation, 8 m of algae liquid was obtained. 3 , algae body density 0.9×10 5 ind / mL. Phaeodactylum tricornutum was cultivated using the three-stage culture method at a temperature of 20°C with ventilation. After 12 days of cultivation, 4m of Phaeodactylum tricornutum liquid was obtained. 3 body with a density of 1.43×10 4 ind / mL.
[0027] Within 24 hours before the land-based test, 7 m 3 and Phaeodactylum tricornutum algae li...
Embodiment 2
[0033] In spring, Qingdao, detection of organisms larger than 50 µm and species of 10-50 µm in natural seawater met the requirements of three categories and five categories, and the concentrations were 31ind / m 3 and 50ind / mL, the number of heterotrophic bacteria was 1.2×10 2 cfu / mL. Therefore, one species of organisms larger than 50 µm and one species of organisms of 10-50 µm need to be cultivated, and the number of organisms should be supplemented.
[0034] The three-stage cultivation method was used to cultivate Sclerophyllum qingdao at a temperature of 22 °C with ventilation. After 12 days of cultivation, 9 m of algae liquid was obtained.3 , algae body density 1.02×10 5 ind / mL.
[0035] Within 24 hours before the land-based test, 7 m 3 Move it to the culture pool for later use.
[0036] At 30°C, hatch Artemia eggs, and collect Artemia 0.7 m within 24 hours before the land-based experiment using the light method. 3 , with a density of 120 ind / mL, stored at 15°C for late...
Embodiment 3
[0041] Qingdao, autumn, detection of biological species larger than 50 µm in natural seawater does not meet the requirements of three categories and five categories, the concentration is 6ind / m 3 , 10-50 μm biological species meet the requirements of three categories and five categories, the concentration is 8ind / mL, and the number of heterotrophic bacteria is 1.1×10 4 cfu / mL. Therefore, there is no need to adjust the number of heterotrophic bacteria when formulating influent water.
[0042] The three-stage culture method was used to cultivate Sclerophytum qingdao, the culture temperature was 22 ℃, and the air was ventilated. After 15 days of culture, 10 m of algae liquid was obtained. 3 , algae body density 1.18×10 5 ind / mL.
[0043] Within 24 hours before the land-based test, the cultured Selmaphyton qingdao algae liquid was 6 m 3 Move it to the culture pool for later use.
[0044] At 30°C, hatch Artemia eggs, and collect Artemia 0.35 m by light method within 24 hours b...
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