Simulated moving bed and method for extracting three-branch chain amino acid based on simulated moving bed

A technology that simulates moving bed and three branched chains, applied in chemical instruments and methods, preparation and application of organic compounds, etc., can solve the problems of insufficient sewage discharge, low production efficiency, high separation efficiency, etc., and save water, electricity and labor Consumption, improve production efficiency, high activity effect

Active Publication Date: 2015-07-01
XINJIANG FUFENG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of three branched chain amino acids generally adopt sedimentation method, ion exchange method and full-membrane method, etc., which are subject to many restrictions in their separation. However, the separation method of simulated moving bed chromatography is easy to realize automation, no regeneration is required, low energy consumption and high separation efficiency. High and adaptable, the purity of three branched-chain amino acids in the extract after separation by simulated moving bed chromatography reaches more than 95%, the sewage discharge is less than one-third of the traditional process, and it is easy to handle
In the amino acid production process, the subsequent refining treatment is also very important, which determines the quality of the product. The traditional drying method is to use the double cone drying method. This method is intermittent, with low production efficiency and high energy consumption. The chemical bed drying method solves these problems, not only improves production efficiency, saves water, electricity, steam and labor consumption, but also greatly reduces production costs

Method used

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  • Simulated moving bed and method for extracting three-branch chain amino acid based on simulated moving bed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of method utilizing simulated moving bed and fluidized bed to extract L-leucine, it comprises the steps:

[0021] 1) Filtrate the L-leucine fermentation liquid that has been fermented through a ceramic membrane to remove bacterial protein to obtain a filtrate; the pore size of the ceramic membrane is 50 nm; the above-mentioned filtrate is concentrated to 1 / 2 volume by a four-effect evaporator , which is the mother liquor;

[0022] 2) Separating the mother liquor in step 1) through sequential simulated moving bed chromatography to obtain a raffinate containing extract and impurities; the conditions and parameters of simulated moving bed chromatography separation are: the eluent is pure water, and the constant temperature is maintained at 60°C , The simulated moving bed chromatographic column is 6 columns, which can be switched in different areas of the column through the dual-channel automatic valve, and the resin filled in the column is a hydrophobic resin.

[0...

Embodiment 2

[0029] A kind of method utilizing simulated moving bed and fluidized bed to extract L-leucine, it comprises the steps:

[0030] 1) The fermented liquid that has been fermented is filtered through a ceramic membrane to remove bacterial protein to obtain a filtrate; the pore size of the ceramic membrane is 70nm; the above-mentioned filtrate is concentrated to 1 / 2 volume by a four-effect evaporator, which is the mother liquor;

[0031] 2) Separating the mother liquor in step 1) through sequential simulated moving bed chromatography to obtain a raffinate containing extract and impurities. The separation conditions and parameters of the simulated moving bed chromatography are as follows: the eluent is pure water, kept at a constant temperature of 60°C, the simulated moving bed chromatographic column is 8 columns, and the dual-channel automatic valve is used to switch between different areas of the column. It is a hydrophobic resin.

[0032] 3) Decolorize the extract described in s...

Embodiment 3

[0041] Fertilizer effect test of fertilizer of the present invention

[0042] 1. Fertilizer experiment for planting sorghum:

[0043] The control group adopts nitrogen, phosphorus and potassium compound fertilizer (nitrogen 15, phosphorus 15, potassium 15), and the experimental group adopts the compound fertilizer prepared by the embodiment of the present invention 1. The soil quality and planting conditions of the test field are exactly the same, and the area is 10 mu; 50kg / mu; specific test results are shown in Table 1:

[0044] Table 1

[0045]

[0046] Note: the compound fertilizer is calculated according to 2.7 yuan / kg, the organic fertilizer of the present invention is calculated according to 2.2 yuan / kg, and the sorghum is calculated according to 3.0 yuan / kg.

[0047] Second, planting sweet potato fertilizer experiment:

[0048] Set up two treatment test fields, both with an area of ​​10 mu, which are the control group and the experimental group. The control grou...

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Abstract

The invention belongs to the technical field of biological fermentation and discloses a simulated moving bed and a method for extracting a three-branch chain amino acid based on the simulated moving bed. The method comprises: performing micro-filtration membrane filtration and four-effect concentration to obtain a sequential simulated moving bed chromatography, separating, decoloring and concentrating and crystalizing the mother liquor, drying the moving bed, preparing a fertilizer by use of wastewater and the like. The method is capable of reducing wastewater emission and energy consumption, and the wastewater also can be used for producing the fertilizer; and double benefits can be achieved.

Description

technical field [0001] The invention relates to a method for extracting three branched-chain amino acids by using a simulated moving bed and a fluidized bed, and belongs to the technical field of biological fermentation. Background technique [0002] The three branched chain amino acids include L-leucine, L-isoleucine and L-valine, all of which are essential amino acids for the human body, and are also very important three amino acids among the 20 amino acids that make up proteins. [0003] At present, the extraction methods of three branched chain amino acids generally adopt sedimentation method, ion exchange method and full-membrane method, etc., which are subject to many restrictions in their separation. However, the separation method of simulated moving bed chromatography is easy to realize automation, no regeneration is required, low energy consumption and high separation efficiency. High and adaptable, the purity of three branched-chain amino acids in the extract reach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/08C07C227/40C05G1/06
Inventor 郭英熙马杰希董爽沈伟伟
Owner XINJIANG FUFENG BIOTECH
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