Refractive index fluorescence microscope

Abstract

A refractive index fluorescence microscope with a refractive index measuring function comprises a laser device 1, a polaroid 2, a long-working-distance object lens 3, a trapezoidal prism 4, a sample 5, a fluorescence detecting device 6 and a detector 7. The prism is a trapezoidal column, a trapezoidal face of the prism is vertically placed, a longer bottom of the trapezoidal face is positioned above, the sample is positioned on a rectangular face above the prism, one rectangular side face of the prism is perpendicular to optical axis of excitation light, the long-working-distance object lens is arranged between the prism and the polaroid, the laser device, the polaroid and the long-working-distance object lens are positioned on a same straight line, and the other rectangular bevel of the prism is parallel to the surface of the detector. The refractive index fluorescence microscope can measure refractive index of a current observed area under excitation wavelength while detecting fluorescence information of a measured object, and is simple in structure, simple in operation and suitable for popularization and use or directly using existing conditions of laboratories for modification.

Description

technical field [0001] The invention relates to the technical field of fluorescence observation instruments, in particular to a fluorescence microscope with the function of measuring the refractive index. Background technique [0002] Fluorescence microscope is a special tool for fluorescence detection, which is a high-tech instrument. Fluorescence microscope irradiates the object to be inspected with excitation light to make it emit fluorescence, and then observe the shape and location of the object under the microscope. Fluorescence microscope is used to study intracellular Fluorescence microscopy is one of the tools for qualitative and quantitative research on substances such as absorption, transportation, distribution and positioning of chemical substances. [0003] In actual work, it is often necessary to measure some physical and chemical indicators of the analyte, such as the refractive index, while observing. However, there is no equipment on the market that can meas...

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Problems solved by technology

[0004] Chinese patent, A Fluorescent Microscope (Application No.: 201410258631.3) discloses a fluorescent microscope, including a frame, a stage and a base, the frame, the stage and the base are arranged sequentially from top to bottom, and the frame More than one adjustment knob is provided on the side of the body, the lower part of the frame body is provided with an objective lens, the stage is located directly below the objective lens, and the bottom of the stage is sequentially provided with a condenser, a diaphragm and a fixed A bracket, an LED fluorescent excitation light source is installed in the middle of the base, the LED fluorescent excitation light source is facing the middle of the stage, and more than one mirror rotating frame is arranged around the LED fluorescent excitation light source. The LED fluorescent excitation light source includes a lampshade, the lampshade is provided with a shielding cover, the shielding cover is movably connected with the LED fluorescent excitation light source, and reflectors are installed on the rotating frames of each reflector; this technical solution can strengthen the fluorescence The observation effect of the microscope, but its disadvantages also include: open-air observation, it is impossible to observe some objects with similar wavelengths of excitation light and sunlight, and it is impossible to measure the refractive index of the excitation part of the object to be measured, etc.

[0005] Chinese patent, laser-excited fluorescence microscopy (application number: 200880112675.8) discloses a technical solution, which mentions the use of laser light as excitation light, but does not involve the problem of refractive index measurement

[0006] In 2012, our school applied for a Chinese patent on the measurement of the refractive index of materials. The method of focusing total internal reflection to measure the distribution of refractive index of materials (application number: 201210062142.1) provides a method for measuring the distribution of refractive index of materials. After the specific realization, the equipment for measuring the refractive index distribution of the material can be obtained, which can be installed on the fluorescence microscope, but this technology cannot be simply copied to the field of fluorescence luminescence refractive index measurement

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