Image acquisition device and image acquisition method

Abstract

In an image acquisition device (M), an optical path length difference changing means, which changes an optical path length difference between a first optical path (L1) and a second optical path (L2), is provided. A focus position for capturing images via a first imaging device (18) changes (moves) in the depth direction of a sample (S) according to the optical path length difference, which is changed by the optical path length difference changing means. Consequently, each time the optical path length difference changing means changes the optical path length difference on the basis of a prescribed target focus interval, a stage control means scans a stage (1) while a focus control means controls the focus position for capturing images via the first imaging device (18), and, by acquiring first images via the first imaging device (18), a Z stack image comprising a plurality of first images can easily be acquired in the depth direction of the sample (S) according to changes in the optical path length difference.

Description

technical field [0001] The present invention relates to an image acquisition device used for image acquisition of a sample or the like and an image acquisition method thereof. Background technique [0002] As an image acquisition device, there is, for example, a virtual microscope device that divides an imaging region of a sample into a plurality of regions in advance, performs high-magnification imaging of each divided region, and synthesizes them. In the image acquisition of such a virtual microscope, conventionally, the imaging conditions when acquiring an image of a sample such as a living body sample have set focus mapping (mapping) that targets the entire area of ​​the sample, and perform focus mapping based on The image of the sample is acquired while controlling the focus. [0003] To create a focus map, first, the entire sample is acquired as a macroscopic image using an image acquisition device equipped with a macroscopic optical system. Next, the imaging range o...

AI Technical Summary

Problems solved by technology

[0004] However, when creating such a focus map, there is a problem that processing takes time

In addition, if the interval or the number of focal points to be obtained is suppressed, the time required for processing is shortened, but in this case, there is a problem that the focus accuracy is reduced.

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