Cadmium strongly inducible promoter cds2 and its application

A promoter and induced expression technology, which is applied in the field of biotechnology and plant genetic engineering, can solve the problems of cadmium measurement interference, etc., and achieve the effect of high accuracy, high detection sensitivity and low detection limit
CN104762302BActive Publication Date: 2019-08-06RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Publication Date
2019-08-06

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Abstract

The invention discloses a plant gene sequence. The present invention isolates, clones and identifies a rice-specific gene promoter Cd S2 that is strongly induced by cadmium (Cd) from Nipponbare rice, and treats the obtained transgenic rice with cadmium (Cd). It is found through GUS chemical staining that after the treatment The transgenic plants had obvious staining in various parts. And through the RT-qPCR analysis of the expression of Gus gene before and after treatment, it was found that after cadmium (Cd) treatment, the expression of Gus gene in transgenic plants was 887.9 times that of before treatment, reaching 4 times the activity of the constitutive promoter ACTIN. The promoter can be used to reflect the intensity of cadmium pollution in soil through its expression level in rice. Its characteristics of simple operation, low detection limit, trace sensitivity and high accuracy are of great significance to the study of environmental relations and the improvement of ecological environment. , play an important role in sustainable development, and can actively analyze the principles of pollution formation, and further provide valuable experience for their avoidance and controllable transformation.
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Description

technical field

[0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant cadmium-inducible expression promoter and its expression and application in rice. The promoter can drive the expression of a target gene in a plant under cadmium-induced conditions in a rice transgenic regulation system, and It can be used to determine the cadmium content in soil. Background technique

[0002] With the rapid development of modern industrial and agricultural production, the industrial "three wastes", the amount of agricultural chemical fertilizers, and solid waste continue to increase, resulting in a sharp increase in the content of heavy metals in the soil. The problem of heavy metal pollution is becoming more and more serious, and has become one of the main problems worldwide (Deng Xiangxiong et al., 2009). Cadmium (Cd) is one of the most toxic heavy metals. Compared with other non-esse...

Claims

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