A kind of method utilizing alkaline protease and keratinase to prepare feather protein powder

A technology of feather protein powder and keratinase, which is applied in the direction of protein food processing, protein food ingredients, applications, etc., can solve the problems of reducing the scope of application, and achieve the effects of reducing production costs, wide pH range, and low enzymolysis temperature

Inactive Publication Date: 2018-08-31
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, due to the differences in the enzymatic hydrolysis sites of different proteases, keratinase and neutral protease were used to interpret feathers, and amino acids were not detected in the enzymatic solution, which greatly reduced its application range.

Method used

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  • A kind of method utilizing alkaline protease and keratinase to prepare feather protein powder
  • A kind of method utilizing alkaline protease and keratinase to prepare feather protein powder
  • A kind of method utilizing alkaline protease and keratinase to prepare feather protein powder

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Experimental program
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Embodiment

[0023] one. Materials and methods

[0024] Material

[0025] Chicken and duck feathers (obtained from poultry slaughterhouse), keratinase: 100000U / g (Jinan Nuoneng Bioengineering Co., Ltd.), alkaline protease: 200000U / g (Jinan Nuoneng Bioengineering Co., Ltd.), 1mol / L NaOH solution, 1mol / L HCL solution, distilled water, Coomassie Brilliant Blue reagent (weigh 100mg Coomassie Brilliant Blue G-250 and dissolve it in 50ml 95% ethanol, add 100mL85% phosphoric acid, add distilled water to dilute to 1000mL.), protein standard solution (crystallized bovine serum Protein, the protein nitrogen content was determined by the micro-Kjeldahl method in advance, and a protein standard solution of 100 μg / mL was prepared according to its purity.), pepsin (the actual titer of protease is 1:3000).

[0026] 2. Main equipment

[0027] main instrument

[0028] 18 250mL Erlenmeyer flasks, pipette (10mL), test tube and test tube rack, volumetric flask (50mL), mortar, 100 mesh standard sieve, drop...

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Abstract

The invention relates to the technical field of animal feed processing, in particular to a method for preparing feather protein powder from alkaline protease / keratinase. Poultry feathers are taken as raw materials, and the method for preparing the feather protein powder from the alkaline protease / keratinase is characterized by comprising steps as follows: (1) preparation of feather powder; (2) enzymolysis; (3) filtration and drying. The technology is simple, the technology application field is broadened, the enzymolysis temperature is low, the pH action range is wide, the enzymolysis cost is low, the enzymolysis efficiency is high, the content of soluble protein of an enzymolysis supernatant is higher, meanwhile, 11 amino acid components with higher content are contained, phenylalanine, isoleucine, leucine and valine are amino acids essential to human bodies, amino acid products and feed additive protein can be processed, the production cost is reduced, and the comprehensive economic benefit is increased.

Description

technical field [0001] The invention relates to the technical field of animal feed processing, in particular to a method for preparing feather protein powder by using alkaline protease and keratinase. Background technique [0002] Feather is one of the important resources in our country. The output is very rich, with an annual output of more than one million tons. It is composed of keratin with a very strong double bond structure. Its chemical composition is the same as that of ordinary proteins, but its molecular shape is similar to that of fibers and its structure is tight It is solid and dense, and it is difficult to be digested and absorbed by animals. It needs to be processed by physical or chemical methods to become a protein that can be digested and absorbed by animals. [0003] The air explosion enzymatic hydrolysis method is used to prepare feather protein powder. This method has complicated procedures and potential safety hazards. On the other hand, the amino acid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/10A23J3/34
Inventor 郝鲁江许艳蕊冯昱畅张涛卢晓平范秋苹
Owner QILU UNIV OF TECH
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