Method for enriching gene 56 target region based on multiple probes

Abstract

The invention discloses a method for enriching a gene 56 target region based on multiple probes. The method comprises the following steps: designing a capture probe library, and enriching a target gene sequence by adopting a probe capture mode in a DNA level so as to detect all variation types of target genes such as mutation, deficiency, insertion, fusion and copy number amplification. By adopting the method disclosed by the invention, the defects that only the mutation and deficiency of the target genes can be detected and the fusion cannot be detected when the target gene sequence is enriched by virtue of a PCR method in a DNA level at present can be overcome.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for enriching 56 gene target regions based on multiple probes. Background technique [0002] Advances in the understanding of the biology of NSCLC (non-small cell lung cancer) over the past few years have led to the identification and potential therapeutic targets of molecular events critical for malignant transformation and cancer cell survival. In recent years, targeted therapy drugs for non-small cell lung cancer have progressed rapidly. FDA-approved EGFR small-molecule inhibitors gefitinib and erlotinib, ALK small-molecule inhibitors crizotinib and Cereb Tini et al. It is worth noting that these targeted therapy drugs are only effective for people who carry certain gene mutations. For example, patients with non-small cell lung cancer carrying EGFR L858R or exon 19 deletion mutations are sensitive to first-generation EGFR small molecule inhibitors. NSC...

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Problems solved by technology

For these patients, the method of sequential detection of molecular target gene variation will cause some gene v

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