Tongue sole molecular marker auxiliary sex control method

A molecular marker-assisted, half-smooth tongue sole technology, applied in botany equipment and methods, biochemical equipment and methods, animal husbandry, etc.

Inactive Publication Date: 2008-07-23
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the sex control technology of half smooth tongue sole, especially the use of molecular marker technology to increase the feminization ratio of half smooth tongue sole fry, that is, molecular marker assisted sex control technology.

Method used

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  • Tongue sole molecular marker auxiliary sex control method
  • Tongue sole molecular marker auxiliary sex control method
  • Tongue sole molecular marker auxiliary sex control method

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Embodiment Construction

[0045] Below, the technical content of the present invention will be described in detail in conjunction with the accompanying drawings through the embodiment of "half smooth tongue sole molecular marker assisted sex control method":

[0046] The present invention comprises four steps: 1) Screening and cloning of female-specific AFLP markers of tongue sole; 2) Establishment of a PCR method for genetic sex identification of tongue sole; 3) Preparation of pseudo-male of tongue-sole; 4) Production of pseudo-male of tongue-sole semi-smoothness with increased feminization ratio Semi-smooth tongue sole fry.

[0047] 1) Screening and cloning of female-specific AFLP markers in tongue sole:

[0048] Including: 1. Screening of female-specific AFLP molecular markers; 2. Cloning and sequence analysis of female-specific AFLP molecular markers;

[0049] 1. Screening of female-specific AFLP molecular markers:

[0050] Use conventional methods to extract genomic DNA from the blood or fin ray...

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Abstract

The invention relates to a tongue sole molecule marker auxiliary sex control method, comprising the following steps: screening and clone of the female tongue sole specific AFLP marker, a construction of tongue sole genetic sex identification PCR method, preparation of pseudo male tongue sole, and feminization fingerling producing method utilizing pseudo male tongue sole; the female tongue sole specific AFLP marker is screened; the pseudo male tongue sole stimulation method and the pseudo male tongue sole identification method utilizing female specific molecule marker are constructed. The tongue sole molecule marker auxiliary sex control method firstly constructs the method that adopts the AFLP molecule marker and the sex reversing technique to control the sex of tongue sole and further to raise the feminization fry ratio, wherein, the ratio of the female fish in the tongue sole fries is increased by 20 to 25%. The tongue sole molecule marker auxiliary sex control method has the advantages of high efficiency, high accuracy, good reliability, important application value in the sex control and unisexual fingerling production of tongue sole, and potential application in the sex control and unisexual fingerling production of other fish.

Description

technical field [0001] The invention belongs to the technical field of fish breeding and cultivation, and relates to a method for increasing the proportion of female fry in half-smooth tongue sole fry by means of molecular markers. Background technique [0002] Half-smooth tongue sole (Cynoglossus semiliaevis) is a rare and economical seawater fish endemic to the coast of my country, and it is also one of the main species of seawater fish farming. Because of its delicious taste, tender meat and rich nutrition, its market value is extremely high, and its breeding prospect is very broad. The aquaculture output is currently nearly 10,000 tons, and the price is expensive. The market price reaches about 150 yuan per 500 grams. The current annual output value of semi-smooth tongue sole aquaculture is about 1.5 billion yuan. However, the latest research shows that there is a huge difference in the growth rate between females and males of half-smooth tongue sole. The females are 3-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/12A01K61/00
CPCY02A40/81
Inventor 陈松林邓思平田永胜马洪雨徐建勇杨景峰季相山邓寒翟介明刘寿堂
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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