Method for determining multiple illegally added chemicals in liquid health food at same time
A health food and illegally added technology, which is applied in the field of medical testing, can solve the problems of affecting health and less screening methods, and achieve the effect of fast, effective and high-throughput analysis
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[0019] 1 Materials and methods
[0020] 1.1 Materials
[0021] 1.1.1 Sample source
[0022] The supplementary memory-improving health food comes from market samples.
[0023] 1.1.2 Main instruments and reagents
[0024] Agilent 1260 high performance liquid chromatography (diode array detector, Agilent, USA), XA205DU electronic analytical balance (Mettler, Switzerland), Classic UVF ultrapure water device (ELGA, UK), P300H ultrasonic cleaner (Elma, Germany) , Sigma 3K15 high-speed centrifuge (Sartorius, Germany), C18-SPE column (60mg / 3mL, Tianjin Bona Agel Technology Co., Ltd.).
[0025] Donepezil, huperzine A, edaravone, betahistine, nicotinic acid zantino, piracetam, galantamine, caffeine, aniracetam, flumazenil, estriol, The reference substances of flunarizine, naproxen, diethylstilbestrol, nimodipine, almitrigine, and diclofenac sodium were all purchased from China National Institutes for Food and Drug Control, with a purity of ≥98.5%; methanol and acetonitrile (liquid q...
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