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Application of nano silver in preparation of medicine for increasing retention of radioiodine in tumor cells

A technology of radioactive iodine and tumor cells, which is applied in the field of medicine, can solve the problems of limited development, insignificant anti-cancer effect of radioactive iodine, inability to effectively increase iodine retention, etc., and achieve the effect of improving the effect and efficiency

Inactive Publication Date: 2015-08-12
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] None of the current technologies can effectively increase the retention of iodine in cells, and the anticancer effect of radioactive iodine is not significant, which limits the development of radioactive iodine in the field of anticancer drugs

Method used

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  • Application of nano silver in preparation of medicine for increasing retention of radioiodine in tumor cells
  • Application of nano silver in preparation of medicine for increasing retention of radioiodine in tumor cells
  • Application of nano silver in preparation of medicine for increasing retention of radioiodine in tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of polyamide-amine (PAMAM)-silver ions

[0020] PAMAM 10*-5mol / L The concentration of silver nitrate is 0.01mol / L, according to the molar ratio of PAMAM and silver nitrate is 1:30, add it into the centrifuge tube to avoid light, and react on the shaker at room temperature for 5 minutes; the color of the solution changes from yellow to brown, and the ultraviolet Spectral analysis and scanning electron microscopy ( figure 1 ,2).

[0021] (2) Amine-amine (PAMAM)-silver ions into the cell

[0022] The synthesized PAMAM-Ag + The solution was added to the 24-well culture dish incubated with lung cancer cell A549-NIS transfected cells, so that the final concentration of PAMAM in the culture dish was 10 -7 mol / L; incubate the culture dish in a 37°C incubator for 2-4 hours as the experimental group; at the same time, the lung cancer cells A549 as the control group 2. Observation of PAMAM-Ag by TEM + into the cell ( image 3 ); the content of intracellular s...

Embodiment 2

[0030] (1) Preparation of polyethylimide (PEI)-silver ions

[0031] The concentration of PEI is 1.5X10*-4mol / L, the concentration of silver nitrate is 0.01mol / L, the volume ratio of PEI and silver nitrate is 1:1, add it to the centrifuge tube and react at room temperature for 5 minutes.

[0032] (2) Polyethylimide (PEI)-silver ions enter the cell

[0033] The synthesized PEI-Ag + The solution was added to the 24-well culture dish incubated with thyroid cancer cell K1-NIS transfected cells, so that the final concentration of PEI in the culture dish was 10 -7 mol / L; incubate the culture dish in a 37°C incubator for 2-4 hours as the experimental group; at the same time, treat thyroid cancer cell K1 in the same way as the control group 1.

[0034] (3) Papillary thyroid cancer cell K1 transfected with NIS gene and expressing NIS protein absorbs radioactive iodine into the cells through NIS

[0035] After drawing out the solution in the cell culture dish, wash the cells twice wit...

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Abstract

The invention discloses an application of nano silver in preparation of a medicine for increasing retention of radioiodine in tumor cells and an application of drug combination of the nano silver and iodine in preparation of radioactive therapy drugs for tumor. The application is advantaged in that by means of increasing the retention of the radioiodine in tumor cells, comprising low-differentiated thyroid cancer cells, or non-thyroid tumor cells transfected with NIS gene and having NIS protein expression, thereby increasing the targeted therapy efficiency of the radioiodine. The radioiodine and silver ions are formed into a nano silver iodide insoluble substance in cells, thereby retarding outflow of the radioiodine from cells, and further improving radioactive therapy effects on tumors.

Description

technical field [0001] The invention belongs to the field of medicine, and more specifically relates to the application of nano-silver in the preparation of retention medicines for increasing radioactive iodine in tumor cells. Background technique [0002] The current method to increase the retention of radioactive iodine in cells is mainly to increase the uptake of iodine by the cells by regulating the amount of intracellular sodium iodide transporter (NIS) or to strengthen the organicization of iodine in cells to reduce the outflow of iodine. [0003] None of the current technologies can effectively increase the retention of iodine in cells, and the anticancer effect of radioactive iodine is not significant, which limits the development of radioactive iodine in the field of anticancer drugs. If the cell efflux of iodine can be effectively blocked, the therapeutic effect of radioactive iodine on tumor cells can be greatly increased. Contents of the invention [0004] The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/06A61K47/48A61P35/00A61K101/02
Inventor 李培勇曹妍
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE