Application of nano-silver in the preparation of drugs that increase the retention of radioactive iodine in tumor cells
A tumor cell and radioactive iodine technology, applied in the field of medicine, can solve the problems of limited development, insignificant anti-cancer effect of radioactive iodine, inability to effectively increase iodine retention, etc., and achieve the effect of improving efficiency and improving effect
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Embodiment 1
[0019] (1) Preparation of polyamide-amine (PAMAM)-silver ions
[0020] PAMAM 10*-5mol / L silver nitrate concentration 0.01mol / L, according to the molar ratio of PAMAM and silver nitrate of 1:30, add it to a centrifuge tube to avoid light, and react at room temperature for 5 minutes on a shaking table; the color of the solution changes from yellow to brown, and the ultraviolet Spectral analysis and scanning electron microscopy ( figure 1 ,2).
[0021] (2) Amine-amine (PAMAM)-silver ions enter cells
[0022] The synthesized PAMAM-Ag + The solution was added to a 24-well culture dish incubating lung cancer cells A549-NIS transfected cells, so that the final concentration of PAMAM in the culture dish was 10 -7 mol / L; the culture dish was incubated in a 37°C incubator for 2-4 hours as the experimental group; at the same time, lung cancer cells A549 were used as the control group 2. Observation of PAMAM-Ag by TEM + into the cell ( image 3 ); the content of intracellular silver...
Embodiment 2
[0030] (1) Preparation of polyacetimide (PEI)-silver ions
[0031] The concentration of PEI is 1.5X10*-4mol / L, the concentration of silver nitrate is 0.01mol / L, and the volume ratio of PEI and silver nitrate is 1:1, and then added to a centrifuge tube for reaction at room temperature for 5 minutes.
[0032] (2) Polyacetimide (PEI)-silver ions enter cells
[0033] The synthesized PEI-Ag + The solution was added to a 24-well culture dish incubating thyroid cancer cell K1-NIS transfected cells, so that the final concentration of PEI in the culture dish was 10 -7 mol / L; the culture dish was incubated in a 37°C incubator for 2-4 hours as an experimental group; at the same time, thyroid cancer cells K1 were treated in the same way as a control group 1.
[0034] (3) The papillary thyroid cancer cell K1 transfected with NIS gene and expressed NIS protein absorbs radioactive iodine into the cells through NIS
[0035] After the solution in the cell culture dish was withdrawn, the cel...
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