Glutathione-responsive nanoprobe for tumor imaging and targeted collaborative treatment and construction method of glutathione-responsive nanoprobe
A glutathione and tumor imaging technology, which is applied in chemical instruments and methods, antineoplastic drugs, preparations for in vivo experiments, etc., can solve the problems of single signal dependence analysis and interference of optical sensors
Active Publication Date: 2021-09-10
QINGDAO UNIV OF SCI & TECH
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Problems solved by technology
[0004] Current optical sensors mainly rely on a single signal-dependent analysis and are susceptible to interference from some substances
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Embodiment 1
[0029] In this embodiment, Cy3 is used as the dual-mode imaging group, and Ce6 is used as the photosensitizer.
[0030] 1. Synthesis of gold nanoparticles
[0031]Take 2L of ethanol, then weigh 500g of potassium hydroxide (KOH), dissolve KOH in 2L of ethanol, and make it into an alkali tank for subsequent experiments. Add a certain amount of ethanol and KOH every 10 days. In this paper, AuNPs are used as the carrier, and its synthesis method is prepared by the traditional citric acid reduction method (also called one-step reduction method). The reducing sodium citrate can convert Au in chloroauric acid solution 3+ After being reduced to Au simple substance, the Au simple substance will further aggregate and grow, and then aggregate into gold nanoparticles with different particle sizes under the control of sodium citrate (controlling the volume ratio of sodium citrate and chloroauric acid). All glassware and magnets used in the preparation process were first soaked in alkali ...
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The invention discloses a glutathione-responsive nanoprobe for tumor imaging and targeted collaborative treatment and a construction method of the glutathione-responsive nanoprobe. The glutathione-responsive nanoprobe comprises a gold nanoparticle and DNA Y-motif, wherein the DNA Y-motif comprises DNA S1, DNA S2 and MUC1 aptamer DNA S3; the DNA sequence S1 is complementarily paired and hybridized with the DNA sequence S2 and the MUC1 aptamer DNA S3 to form a Y-shaped structure; a sulfydryl group at the 5' end of the S1 sequence is connected to the surface of the gold nanoparticle to form AuNP Y-motif; the 5' end of the S2 sequence modifies a photosensitizer; the 3' end of the S2 sequence modifies a dual-mode imaging group and is close to the surface of the gold nanoparticle; and fluorescence-surface enhanced Raman scattering dual-mode tumor cell imaging is realized by adjusting a distance between the dual-mode imaging group and the gold nanoparticle. According to the method, an aptamer is used as a target, and the nanoprobe carrying a chemotherapeutic drug and the photosensitizer is constructed by using an endogenous substance DNA, so the collaborative treatment of CT-PDT is realized.
Description
Technical field: [0001] The invention belongs to the field of tumor cell synergistic therapy and spectral imaging, and specifically relates to a glutathione-responsive nanoprobe for tumor imaging and targeted synergistic therapy and its construction method, which realizes the co-delivery of chemotherapeutic drugs and photosensitizers Synergistic treatment of chemotherapy (CT) and photodynamic therapy (PDT) and dual-spectrum (fluorescence spectroscopy, surface-enhanced Raman scattering spectroscopy) imaging analysis of tumor cells. Background technique: [0002] Glutathione (GSH) is a kind of sulfhydryl compound, and abnormal changes of GSH content in cells are directly related to the occurrence of some diseases, such as tumors, neurological diseases, AIDS and so on. GSH has become a recognized tumor cell marker, and it is significantly higher than normal cells in many malignant tumors. Therefore, monitoring and imaging research on GSH is an urgent need for early diagnosis o...
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Login to View More IPC IPC(8): C09K11/06A61K31/704A61K41/00A61K47/54A61K49/00A61P35/00
CPCC09K11/06A61K31/704A61K41/0071A61K47/549A61P35/00A61K49/0002A61K49/0032A61K49/0052C09K2211/188A61K2300/00
Inventor 付卓林李英任春年苏聪
Owner QINGDAO UNIV OF SCI & TECH