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A kind of induced pluripotent stem cell culture medium, application and culture method

A pluripotent stem cell and culture method technology, applied in the field of induced pluripotent stem cell culture medium, can solve the problems of reducing the application value and safety of IPS cells, slow proliferation of IPS cells, etc. The risk of animal-derived hospitals and the effect of improving safety

Active Publication Date: 2018-07-10
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, IPS cells are mostly cultured in media containing fetal bovine serum (FBS), such as DMEM / F12+20% FBS, but FBS originates from animals and may contain animal-derived pathogens, which reduces the clinical application of IPS cells value and security
Although a few manufacturers have developed a serum-free IPS cell culture medium, the proliferation of IPS cells cultured in this medium is slow

Method used

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  • A kind of induced pluripotent stem cell culture medium, application and culture method
  • A kind of induced pluripotent stem cell culture medium, application and culture method
  • A kind of induced pluripotent stem cell culture medium, application and culture method

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Experimental program
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Embodiment 1

[0037] The stem cell serum-free medium is composed of the following components:

[0038]

[0039]

Embodiment 2

[0041]

[0042]

Embodiment 3

[0044] ingredient name

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Abstract

The invention provides an induced pluripotent stem cell culture medium for the field of biotechnology as well as application and a cultivation method thereof. IMDM / F12 is used as a basal culture medium. Each 500mL of IMDM / F12 basal culture medium comprises recombinant human insulin, recombination transferrin, vitamin C, human albumin, bFGF, PDGF-bb, EGF, IGF-1, TGF-beta, fibronectin, Fetuin, Betacellulin, beta-mercaptoethanol and Thiazovivin. As animal serum is completely unavailable in the induced pluripotent stem cell culture medium provided by the invention, the risk of carrying animal-derived pathogene is effectively avoided, and the safety of the induced pluripotent stem cell in clinical application is improved. The culture medium provided by the invention can enable the induced pluripotent stem cells to proliferate quickly and has very high application values in clinical practice.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an induced pluripotent stem cell culture medium, application and culture method. Background technique [0002] Induced pluripotent stem cells (iPS cells) were originally introduced by Japanese Shinya Yamanaka in 2006 using a combination of four transcription factors (Oct4, Sox2, Klf4 and c-Myc) into A cell type similar to embryonic stem cells obtained by reprogramming differentiated somatic cells. Induced pluripotent stem cells can reprogram somatic cells to obtain pluripotent stem cells with embryonic stem cell-like characteristics by introducing inducible genes into somatic cells, also known as dedifferentiation. [0003] Subsequently, different scientists around the world have discovered that other methods can also produce such cells. After the successful establishment of iPS cells, the application of iPS cells to treat diseases is the ultimate goal of people. iPS cells can not...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0735
Inventor 王一飞陈海佳葛啸虎卢瑞珊王小燕李平
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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