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Preparation method of needle mushroom anti-tumor water-soluble composition

A production method and anti-tumor technology, applied in anti-tumor drugs, drug combinations, food preparation, etc., can solve the problems of high cost of purification process, poor effect, and reduced efficacy of purified ingredients, so as to drive economic development and increase additional value, the effect of eliminating pollution

Active Publication Date: 2015-08-19
仙芝科技(福建)股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some of these reports were simply extracted without any steps of purification, and the crude extract was directly used for pharmacological research, and the drug effect was not very satisfactory; some were extracted and purified to obtain a relatively pure certain A class of ingredients, and then conduct pharmacological research, the result is that the efficacy of some purified ingredients is improved, but the cost of complicated purification process is higher; the efficacy of some purified ingredients is reduced instead
Modern pharmacological studies have shown that some Chinese herbal medicines and edible and medicinal fungi in natural products have good tumor-inhibiting effects, but the specific mechanism of action or what kind of substance is working has puzzled us so far. Researchers have found that the real It is the combination of some compound ingredients that has a better anti-tumor effect, but the effect of a single ingredient is not good

Method used

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  • Preparation method of needle mushroom anti-tumor water-soluble composition
  • Preparation method of needle mushroom anti-tumor water-soluble composition
  • Preparation method of needle mushroom anti-tumor water-soluble composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Embodiment 1: a kind of preparation method of Flammulina velutipes anti-tumor water-soluble composition, get fresh Flammulina velutipes fruit body 10kg, add water by weight: solid-liquid ratio 1:5, 95 ℃ of atmospheric pressure extract 3h, stand and cool to room temperature (25 ℃ About), 80-200 mesh filtration, repeated extraction once, standing, cooling, 100 mesh filtration, combined two extracts; the extract was separated by 0.5μm ceramic membrane and 10nm ultrafiltration membrane to obtain ceramic membrane and ultrafiltration membrane The retentate in between; the obtained retentate is concentrated to a concentration of 15mg / mL, and then decolorized by DEAE-52 resin, the solid-liquid ratio of the resin to the concentrated retentate is 1:30, the eluent is distilled water, and the eluate is collected , washed until the absorbance value of the eluate remained unchanged; concentrated and dried by conventional drying techniques to obtain 91 g of Flammulina velutipes anti-tum...

Embodiment 2

[0029] Example 2: Take 10kg of fresh Flammulina velutipes fruiting bodies, add water according to the ratio of material to liquid 1:4, extract at 100°C for 1.5h, let stand and cool to about 20°C, filter with 80-200 mesh, repeat the extraction once, let stand, cool, and filter , combined the two extracts; the extract was separated by 0.1 μm ceramic membrane and 5nm ultrafiltration membrane to obtain the retentate between the ceramic membrane and the ultrafiltration membrane; the obtained retentate was concentrated to a concentration of 20mg / mL, and then subjected to DEAE -52 resin decolorization, the resin solid-to-liquid ratio is 1:25, the eluent is distilled water, collect the eluent, and wash until the absorbance of the eluent remains constant; concentrate and freeze-dry, Flammulina velutipes anti-tumor water-soluble composition 87g .

Embodiment 3

[0030] Embodiment 3: get fresh Flammulina velutipes sporocarp 100kg, add water according to material-liquid ratio 1:3, extract 1.5h at 90 ℃, leave standstill, cool, 120 mesh filter, repeat extraction once, leave standstill, cool, 120 mesh filter, merge two Secondary extraction solution; the extraction solution is separated by 0.1 μm ceramic membrane and 5nm ultrafiltration membrane to obtain the retentate between the ceramic membrane and the ultrafiltration membrane; the obtained membrane retentate is concentrated to a concentration of 15mg / mL, and then passed through DEAE-52 The resin was decolorized, the solid-to-liquid ratio of the resin was 1:35, and the eluent was distilled water. The eluate was collected and washed until the absorbance value of the eluent remained unchanged; concentrated and spray-dried to obtain 780 g of an anti-tumor water-soluble composition of Flammulina velutipes.

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Abstract

The invention belongs to the extraction and preparation methods of functional components of edible mushrooms, and discloses a preparation method of a needle mushroom anti-tumor water-soluble composition. The key points are that the needle mushroom anti-tumor water-soluble composition is prepared by the method including the steps: adding water to fresh needle mushroom fruiting body and heating for extraction; cooling and filtering to obtain crude extract; separating the crude extract by films; collecting an intercepted part between a ceramic film and an ultra-filtration film; collecting eluent after removing color and impurities through resin; and condensing and freeze-drying to obtain the anti-tumor water-soluble composition. The water-soluble composition obtained by the preparation method comprises impurities such as water-soluble polysaccharide, protein and nucleic acid. No organic solvent is carried in the whole separation process, so that pollution from the organic solvent to the environment is removed; and the biological activity of the separated substance is kept to the maximum extent, the technology is simple, convenient, efficient and environment-friendly. The in-vitro cell experiments prove that the needle mushroom anti-tumor water-soluble composition has powerful suppression function on tumor cells of colorectal cancer, liver cancer, gastric cancer and nasopharynx cancer of a human.

Description

technical field [0001] The invention belongs to a method for extracting and preparing functional components of edible fungi, in particular to a method for preparing an anti-tumor water-soluble composition of Flammulina velutipes. Background technique [0002] At present, cancer has become one of the top two causes of human death, seriously endangering human physical and mental health. Although after so many years of hard work, human beings have achieved some gratifying achievements in the fight against cancer, but to truly conquer cancer is there's still a long way to go. In recent years, more and more experts and scholars have turned their attention to natural products, especially edible and medicinal fungi, in order to find out the effects of them that can be used to treat cancer, reduce the toxic and side effects of tumor radiotherapy and chemotherapy, or enhance the body's immunity. It is used as a raw material to develop anti-tumor drugs or health food, which will brin...

Claims

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Application Information

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IPC IPC(8): A61K36/06A23L1/29A23L1/28A61P35/00A23L33/00
Inventor 李晔朱忠敏周岩飞吴长辉姚渭溪
Owner 仙芝科技(福建)股份有限公司
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