Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors

A technology of schizophrenia and neurons, which is applied in the field of GABA neurons composition, can solve the problems of not providing methods for abnormal behaviors of schizophrenia, and achieve the goal of improving risk avoidance, improving misjudgment, and realizing specificity Effect

Active Publication Date: 2015-08-19
SHENZHEN INST OF ADVANCED TECH
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the nucleus accumbens can be used as a target to regulate abnormal behaviors in schizophrenia has not been studied, and the existing technology has not yet provided a method for specifically intervening in the nucleus accumbens to improve abnormal behaviors in schizophrenia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors
  • Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors
  • Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of a lentiviral vector carrying the Syn-ChR2-eYFP plasmid.

[0034] Prepare a lentiviral vector for infecting the nucleus accumbens in living animals, which carries a Syn-ChR2-eYFP plasmid: the plasmid (0.5 μg) (the structure of the fusion plasmid is as follows Figure 1A shown), together with the mixed plasmids pCMVΔR8.74 and pMD2.G. (1.5 μg) related to lentiviral packaging, were transfected into 293FT cells (products of ATCC Company) using liposomes (products of Invitrogen Company), the cells were passaged After 25 passages, a new batch of cell lines needs to be replaced) (6 μL liposomes per 200,000 cells). After 24 hours, the culture medium for culturing 293FT cells was replaced with serum-free DMEM containing 5 mM sodium pyruvate and continued incubation. After 16 hours, the ultracentrifuge was used to rupture the membrane at a speed of 50,000 g, the suspension was passed through a 20% sucrose filter column, and the supernatant was collected. The v...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors. The composition comprises a light-sensation-gene-carrying virus vector for infecting GABAergic neurons in nucleus accumbens and an irradiation device capable of generating blue light, wherein the vector comprises a promoter, a light sensation gene and a green fluorescence labeling gene; the promoter is an Syn promoter; the irradiation device is used for carrying out irradiation control on the infected nucleus accumbens to excite the nucleus accumbens. The composition can accurately and specifically excite GABAergic neurons in nucleus accumbens, and can effectively improve the schizophrenia abnormal behaviors.

Description

technical field [0001] The invention relates to a composition for specifically exciting GABAergic neurons in the nucleus accumbens and its application in improving abnormal behaviors of schizophrenia. Background technique [0002] The epidemiological survey of mental illness in 12 regions in China shows that there are currently about 100 million people in the country suffering from mental illness, and about 16 million people with severe mental illness, and about 250,000 people die by suicide every year. Among the 340 million children and adolescents under the age of 17 in China, about 30 million suffer from emotional disorders and psychological and behavioral problems. Neuropsychiatric diseases currently rank first in the total burden of disease in my country, accounting for one-fifth of all diseases and trauma-induced disabilities and labor losses. According to experts' prediction, the 21st century will be the century of the prevalence of mental illnesses. With the acceler...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/867
CPCA61N5/06C12N15/867
Inventor 屠洁王立平杨帆刘运辉
Owner SHENZHEN INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com