Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors

A technology of schizophrenia and neurons, which is applied in the field of GABA neurons composition, can solve the problems of not providing methods for abnormal behaviors of schizophrenia, and achieve the goal of improving risk avoidance, improving misjudgment, and realizing specificity Effect

Active Publication Date: 2015-08-19
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the nucleus accumbens can be used as a target to regulate abnormal behaviors in schizophrenia has not been studied, and the exis

Method used

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  • Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors
  • Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors
  • Composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of a lentiviral vector carrying the Syn-ChR2-eYFP plasmid.

[0034] Prepare a lentiviral vector for infecting the nucleus accumbens in living animals, which carries a Syn-ChR2-eYFP plasmid: the plasmid (0.5 μg) (the structure of the fusion plasmid is as follows Figure 1A shown), together with the mixed plasmids pCMVΔR8.74 and pMD2.G. (1.5 μg) related to lentiviral packaging, were transfected into 293FT cells (products of ATCC Company) using liposomes (products of Invitrogen Company), the cells were passaged After 25 passages, a new batch of cell lines needs to be replaced) (6 μL liposomes per 200,000 cells). After 24 hours, the culture medium for culturing 293FT cells was replaced with serum-free DMEM containing 5 mM sodium pyruvate and continued incubation. After 16 hours, the ultracentrifuge was used to rupture the membrane at a speed of 50,000 g, the suspension was passed through a 20% sucrose filter column, and the supernatant was collected. The v...

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Abstract

The invention provides a composition capable of specifically exciting GABAergic neurons in nucleus accumbens and application thereof in improving schizophrenia abnormal behaviors. The composition comprises a light-sensation-gene-carrying virus vector for infecting GABAergic neurons in nucleus accumbens and an irradiation device capable of generating blue light, wherein the vector comprises a promoter, a light sensation gene and a green fluorescence labeling gene; the promoter is an Syn promoter; the irradiation device is used for carrying out irradiation control on the infected nucleus accumbens to excite the nucleus accumbens. The composition can accurately and specifically excite GABAergic neurons in nucleus accumbens, and can effectively improve the schizophrenia abnormal behaviors.

Description

technical field [0001] The invention relates to a composition for specifically exciting GABAergic neurons in the nucleus accumbens and its application in improving abnormal behaviors of schizophrenia. Background technique [0002] The epidemiological survey of mental illness in 12 regions in China shows that there are currently about 100 million people in the country suffering from mental illness, and about 16 million people with severe mental illness, and about 250,000 people die by suicide every year. Among the 340 million children and adolescents under the age of 17 in China, about 30 million suffer from emotional disorders and psychological and behavioral problems. Neuropsychiatric diseases currently rank first in the total burden of disease in my country, accounting for one-fifth of all diseases and trauma-induced disabilities and labor losses. According to experts' prediction, the 21st century will be the century of the prevalence of mental illnesses. With the acceler...

Claims

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Application Information

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IPC IPC(8): C12N15/867
CPCA61N5/06C12N15/867
Inventor 屠洁王立平杨帆刘运辉
Owner SHENZHEN INST OF ADVANCED TECH
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