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A kind of preparation method of bacterial cellulose with antibacterial properties

A technology of bacterial cellulose and cellulose, applied in the field of microorganisms, can solve problems such as complicated processes, achieve the effect of simplifying the complicated preparation process and solving the effect of toxicity

Active Publication Date: 2018-02-23
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process goes through multiple steps and the process is complicated
And at present, there is no report on the preparation of bacterial cellulose with antibacterial properties by mixed multiple fermentation methods in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Seed solution preparation: take two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1CCTCC M208149 and put them into a 500mL Erlenmeyer flask containing 50mL liquid activation medium, 28°C, 100r / m , with a stroke of 65mm and shake culture for 36h to obtain seed liquid. (The formula components per liter of liquid activation medium are as follows: 20g of glucose, 5g of peptone, 5g of yeast extract, 2.7g of disodium hydrogen phosphate, 1.15g of citric acid, and the balance is water. After mixing the above ingredients evenly, adjust the pH value 6.0, sterilized for use).

[0020] (2) Fermentation of bacterial cellulose: Take 5mL of seed liquid and add it to 500mL Erlenmeyer flask with 50mL of fermentation medium, 28 ℃ reciprocating shaker shake culture for 36 hours, shaker speed is 60r / m, stroke is 75mm, get fermentation broth. (The fermentation medium contains 20g of glucose per liter, 5g of yeast extract, 10g of peptone, 1g of citric acid, 1.5g of di...

Embodiment 2

[0026] (1) Seed solution preparation: take two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1CCTCC M208149 and put them into a 500mL Erlenmeyer flask containing 50mL liquid activation medium, 28°C, 100r / m , with a stroke of 65 mm and shake culture for 24 hours to obtain seed liquid. (The formula components per liter of liquid activation medium are as follows: 20g of glucose, 5g of peptone, 5g of yeast extract, 2.7g of disodium hydrogen phosphate, 1.15g of citric acid, and the balance is water. After mixing the above ingredients evenly, adjust the pH value 6.0, sterilized for use).

[0027] (2) Fermentation of bacterial cellulose: 5 mL of seed liquid was added to 80 mL of fermentation medium in a 500 mL Erlenmeyer flask, and cultured on a rotary shaker at 28° C. for 18 hours at a speed of 20 r / m to obtain a fermentation liquid. (The fermentation medium contains 40g of glucose per liter, 1g of yeast extract, 8g of peptone, 8g of disodium hydrogen phosphate, 6...

Embodiment 3

[0033] (1) Preparation of seed solution: Take two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1CCTCC M208149 and put them into a 500mL Erlenmeyer flask containing 50mL of liquid activation medium, 32°C, 80r / m , with a stroke of 75 mm and shake culture for 18 hours to obtain seed liquid. (The formula components per liter of liquid activation medium are as follows: 50g of glucose, 10g of peptone, 8g of yeast extract, 1.5g of disodium hydrogen phosphate, 1g of citric acid, and the balance is water. The above-mentioned ingredients are mixed evenly, and the pH value is adjusted to 6.0. for sterilization).

[0034] (2) Fermentation of bacterial cellulose: Take 3mL of seed liquid and add it to 60mL of fermentation medium in a 500mL Erlenmeyer flask, shake and cultivate in a reciprocating shaker at 32°C for 18 hours, the shaker speed is 20r / m, and the stroke is 65mm, to obtain fermentation broth. (The fermentation medium contains 60g of glucose per liter, 0.8g of...

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Abstract

The invention discloses a preparation method of bacterial cellulose with antibacterial properties. Take gluconoacetic acid bacteria GD‑BC‑1 for fermentation and cultivation to obtain a fermentation liquid containing bacterial cellulose, then add bacteria producing antimicrobial peptides to the fermentation liquid, the bacterial cellulose embeds the added bacteria producing antimicrobial peptides and continue Fermentation to obtain bacterial liquid containing cellulose clusters, take out the cellulose clusters, put them into MRS medium for cultivation, then take out the cellulose clusters and rinse with water to obtain bacterial cellulose with antibacterial properties. The present invention wraps bacteria producing antimicrobial peptides in the process of synthesizing bacterial cellulose by mixing multiple fermentation methods. By converting the fermentation medium, the antibacterial peptide bacteria synthesize the antimicrobial peptide and mount it on the bacterial cellulose to make bacterial cellulose with antibacterial properties. The poisonous effect of direct addition of antibacterial agent on bacterial cellulose-producing bacteria is solved. Simplify the complicated preparation process of traditional antibacterial bacterial cellulose.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to a preparation method of bacterial cellulose with antibacterial properties. Background technique: [0002] Bacterial cellulose is a polymer secreted by bacteria. In addition to the characteristics of general cellulose, it also has many unique properties. It has a three-dimensional network structure. Bacterial cellulose has a high ability to bind water; the surface area is larger than that of plant fibers, which has a higher chemical adsorption capacity; better mechanical properties, which have high elasticity, high resilience and high tensile strength. These many advantages make bacterial cellulose a potential as a dressing for wounds. [0003] The ingredient of bacterial cellulose is only cellulose, which has no antibacterial function. In order to make bacterial cellulose have antibacterial function, soaking method and chemical grafting method are currently used. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/04C12P21/00C12N11/12C12N11/04C12R1/02C12R1/46C12R1/245C12R1/01
Inventor 冯劲施庆珊谢小保黄小茉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY