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Monitoring temperature with fluorescence

一种荧光染料、温度控制的技术，应用在校准样本加热装置领域

Active Publication Date: 2015-08-19
UNIV OF UTAH RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since biochemical reactions in PCR are fast and there are many ways to rapidly change the temperature of samples (especially small ones), the factor that limits the consistency of PCR, especially when fast, appears to be accurate temperature measurement

Method used

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  • Monitoring temperature with fluorescence
  • Monitoring temperature with fluorescence
  • Monitoring temperature with fluorescence

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other Embodiment approach

[0113] Other embodiments may use ratios when measuring fluorescence over two (single-dye / dual-color) or even three (single-dye / tri-color) spectral bands, as further described below in documents incorporated herein by reference in their entirety. Discussion: (1) P. Lavieille, F. Lemoine, G. Lavergne, M. Lebouché, Evaporating and combusting droplet temperature measurements using two-colorlaser-induced fluorescence, Exp. Fluids 21 (2001) 45–55; (2) M . Bruchhausen, F. Guillard, F. Lemoine, Instantaneous measurement of two-dimensional temperature distributions by means of two-color planar laser induced fluorescence (PLIF), Exp. Fluids 38 (2005) 123–131; (3) P. Lavieille , A. Delconte, D. Blondel, M. Lebouché, F. Lemoine, Non-intrusive temperature measurements using three-color laser-induced fluorescence, Exp. Fluids 36 (2004) 706–716; and (4) M. Bruchhausen, A. Delconte, Temperature measurements in polydisperse sprays by means of laser-induced fluorescence (LIF) on three spectral ...

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Abstract

Systems, methods, and kits are provided wherein a temperature-sensitive reagent that emits a luminescent signal is used to adjust the identification of the temperature of a sample or to control thermocycling. In various illustrative embodiments, the sample is a PCR mixture.

Description

[0001] Cross References to Related Applications [0002] This application claims priority and benefit to US Provisional Patent Application No. 61 / 711,631, filed October 9, 2012, which is hereby incorporated by reference in its entirety. Background technique [0003] Polymerase chain reaction ("PCR") is a widely used technique in molecular biology. Their name is derived from one of the key components, the DNA polymerase that amplifies DNA strands by enzymatic replication. Generally, PCR uses a thermostable polymerase, deoxynucleoside triphosphates ("dNTPs"), a pair of primers, and template DNA. A single PCR reaction (or cycle) typically involves (1) raising the temperature of the sample to a temperature sufficient to melt or denature double-stranded DNA molecules into single-stranded templates, (2) cooling the sample to allow the DNA primers to bind or anneal to each template, And optionally (3) readjusting the sample temperature to optimize the enzymatic addition of dNTPs to...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01K11/20G01N2021/6439G01N21/6428B01L7/52B01L2300/0636B01L2300/18C12Q1/686C12Q3/00G01K11/32
Inventor L·N·斯坦福C·T·威特沃
Owner UNIV OF UTAH RES FOUND