Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of total flavonoids extract of Pteridophyta

A kind of Auricularia spinosa and its extraction method, which are applied in the fields of medical raw materials, drug combinations, organic chemistry, etc. derived from ferns/filamentous plants

Active Publication Date: 2018-03-27
JIUJIANG UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction process of total plant flavonoids mainly includes reflux extraction, ultrasonic method, microwave method, supercritical fluid extraction method, enzymatic hydrolysis, etc., but the above methods can only obtain crude extracts, and the content of total flavonoids does not exceed 10%. , therefore, to establish a preparation method to obtain a high content of total flavonoids extract of Auricularia thorns, and to provide a basis for further research on the pharmacological activity of flavonoids in Auricularia thorns

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of total flavonoids extract of Pteridophyta
  • A kind of preparation method of total flavonoids extract of Pteridophyta
  • A kind of preparation method of total flavonoids extract of Pteridophyta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of the crude extract of Auricularia thorns is as follows: take 100 g of Auricularia thorns, add concentration of 60% ethanol, add ethanol weight is 20 times of the weight of Auricularia thorns, use ultrasonic extraction, Extraction power is 350W, extract 3 times, 30 minutes each time, combine the extracts, recover ethanol to the utmost, concentrate and dry to obtain the crude extract of Auricularia auricularia.

[0041]The purification method of the total flavonoids extract of Auricularia thorns is as follows: take the crude extract of Auricularia thorns, add water to dissolve it completely, the weight of adding water is 0.5 times the weight of the crude extract of Auricularia thorns, and adjust the pH value If it is 5.0, put it on the polyamide column, wash it with water first, the Molish reaction is negative, stop collecting the washing solution, and discard the washing solution; then elute with 60% ethanol, the hydrochloric acid-magnesium powder...

Embodiment 2

[0043] The preparation method of the crude extract of Auricularia thorns is as follows: take 1000 g of Auricularia thorns, add concentration of 60% ethanol, add ethanol weight is 20 times of the weight of Auricularia thorns, use ultrasonic extraction, Extraction power is 350W, extract 3 times, 30 minutes each time, combine the extracts, recover ethanol to the utmost, concentrate and dry to obtain the crude extract of Auricularia auricularia.

[0044] The purification method of the total flavonoids extract of Auricularia thorns is as follows: take the crude extract of Auricularia thorns, add water to dissolve it completely, the weight of adding water is 0.5 times the weight of the crude extract of Auricularia thorns, and adjust the pH value If it is 5.0, put it on the polyamide column, wash it with water first, the Molish reaction is negative, stop collecting the washing solution, and discard the washing solution; then elute with 60% ethanol, the hydrochloric acid-magnesium powd...

Embodiment 3

[0046] The preparation method of the crude extract of Auricularia thorns is as follows: take 500 g of Auricularia thorns, add concentration of 60% ethanol, add ethanol weight is 20 times of the weight of Auricularia thorns, use ultrasonic extraction, Extraction power is 350W, extract 3 times, 30 minutes each time, combine the extracts, recover ethanol to the utmost, concentrate and dry to obtain the crude extract of Auricularia auricularia.

[0047] The purification method of the total flavonoids extract of Auricularia thorns is as follows: take the crude extract of Auricularia thorns, add water to dissolve it completely, the weight of adding water is 0.5 times the weight of the crude extract of Auricularia thorns, and adjust the pH value If it is 5.0, put it on the polyamide column, wash it with water first, the Molish reaction is negative, stop collecting the washing solution, and discard the washing solution; then elute with 60% ethanol, the hydrochloric acid-magnesium powde...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of the total flavonoids extract of Auricularia thorns, which comprises the extraction method of the crude extract of Auricularia thorns and the total flavonoids extract of Auricularia thorns The purification method, wherein the extraction method of the crude extract of Auricularia thorns is as follows: take Auricula thorns and add ethanol, ultrasonically extract, recover the ethanol from the extract, concentrate and dry, and obtain the crude extract of Auricularia thorns ; Wherein the purification method of the total flavonoids extract of Auricularia thorns is: take the crude extract of Auricularia thorns and add water to dissolve it, adjust the pH value, put it on a polyamide column, wash it with water first, discard the washing solution, and then use the concentration The eluate is eluted with 50%-70% ethanol, and the ethanol is recovered from the eluent, concentrated and dried to obtain the total flavonoids extract of Auricularia auricola. The method is organic and integral, and has the advantages of high extraction rate, simple process, suitability for industrial production, high content of total flavonoids in the extract of Auricularia spinosa and the like.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of total flavonoids extract of Auricularia thorns. Background technique [0002] Arachniodes exilis Ching is a plant of the genus Arachniodes exilis Ching, which is widely distributed in tropical and subtropical humid regions of the world, with the most abundant species in eastern and southeastern Asia. It is mainly distributed in the Yangtze River Basin and beyond in my country In southern China and Shandong, Henan and other provinces, it has the functions of clearing away heat and dampness, antibacterial and anti-inflammatory, and sedative. It is used by folks to treat acute jaundice hepatitis, arthritis, waist and leg pain, dysentery, and burns. Its main chemical components are flavonoids . Studies have shown that the Phryllopsis fern has pharmacological effects including anti-virus, anti-tumor, etc., and the ingredients th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30A61K36/11A61P31/12A61P35/00
Inventor 李辉敏
Owner JIUJIANG UNIV