A method for promoting extracellular expression of target protein and its application

A technology of target protein and protein, which is applied in the field of promoting the extracellular expression of target protein, can solve problems such as unfavorable fermentation regulation and industrial amplification, and achieves the improvement of the ratio of extracellular enzyme activity and production intensity, the improvement of production intensity, and the convenient regulation of enzyme production process. Effect
CN104878033BActive Publication Date: 2018-08-28JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2018-08-28

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Abstract

The invention discloses a method for promoting extracellular expression of target protein and an application of the method, and belongs to the technical field of bioengineering. Bacillus cereus phospholipase C and the target protein (including intracellular location protein and extracellular location protein) are subjected to coexpression in escherichia coli, so that the production intensity of the extracellular location target protein can be improved; extracellular expression of the intracellular location target protein is realized; the production efficiency is improved; the post-extraction technology is simplified; and the method has relatively high academic significance and application value.
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Description

technical field

[0001] The invention relates to a method for promoting the extracellular expression of a target protein and its application, and belongs to the technical field of bioengineering. Background technique

[0002] There are two localization methods of recombinant proteins expressed by genetically engineered bacteria: intracellular localization, with the help of a series of chaperone proteins, the protein synthesized in the ribosome is localized in the cell matrix; extracellular localization, after synthesis in the ribosome, The target protein is transported to the extracellular matrix under the action of its own functional sequence and the secretion system of the host bacteria. Among these two positioning methods, extracellular positioning not only helps protein folding, but also helps to reduce the amount of inclusion bodies formed. At the same time, it can avoid operations such as breaking cells during downstream purification, simplify purification steps, reduce...

Claims

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