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Method of tree species selection for afforestation in lightly saline soil based on biolog Eco microplate

A technology of microplates, tree species, applied in the direction of material analysis, measuring devices, instruments, etc. by optical means

Active Publication Date: 2017-04-05
HEILONGJIANG FOREST ENG & ENVIRONMENT INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the combination of these two techniques and analytical methods for tree species selection in saline soil afforestation

Method used

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  • Method of tree species selection for afforestation in lightly saline soil based on biolog Eco microplate
  • Method of tree species selection for afforestation in lightly saline soil based on biolog Eco microplate
  • Method of tree species selection for afforestation in lightly saline soil based on biolog Eco microplate

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Experimental program
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Embodiment Construction

[0020] (1) Collect rhizosphere soil samples of different afforestation tree species. The collected soil samples were divided into two parts, one was stored in a refrigerator at 4°C for Biolog Eco microplate experiments within 7 days, and the other was air-dried in the shade for the determination of various environmental indicators. (2) Take the rhizosphere soil sample of afforestation tree species to be selected and carry out the Biolog Eco microplate experiment, continuously measure the absorbance value of 590nm / 750nm wavelength for 10 days, and draw the average absorbance value (AWCD) curve, select the curve tending to according to the curve change situation The absorbance value at flat time is used for subsequent experimental analysis, and the data is saved as microbial diversity data in a .csv format file (one row represents a sample, and one column represents a carbon source). (3) Measure the various environmental indicators of the rhizosphere soil samples of different af...

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Abstract

The invention provides a method for selecting tree species for afforestation in lightly saline soil based on the Biolog Eco microplate. It mainly includes: (1) Collecting rhizosphere soil samples of different afforestation tree species; (2) Conducting Biolog Eco microplate experiment and drawing the average absorbance value (AWCD) curve; (3) Determination of environmental indicators of rhizosphere soil samples of different afforestation tree species; (4) ) RDA / CCA ranking analysis. The invention provides a method for selecting afforestation tree species in lightly saline soil based on the Biolog Eco microplate, which is a fast and sensitive method for afforestation tree species selection. It solves the problems of heavy workload, long period, and large capital investment in traditional methods, and fills up the relevant theoretical gaps, thereby laying the foundation for the improvement and utilization of mildly saline soil.

Description

technical field [0001] The invention relates to a method for selecting tree species for afforestation in lightly saline soil. Background technique [0002] Saline soil is a general term for saline soil, alkaline soil and various salinized and alkalized soils. According to different salinity and pH value, saline soil can be divided into mildly saline soil (pH value is 7.1-8.5, salt content is less than 0.3%), moderately saline soil (pH value is 8.5-9.5, Salt content 0.3% ~ 0.6%) and severe saline soil (pH value above 9.5, salt content higher than 0.6%). There are 99.13 million hectares of saline soil in my country. Due to drought, irrational irrigation, overgrazing and other reasons, the area of ​​saline soil is gradually increasing. The improvement and utilization of saline soil has long-term strategic significance. Among them, severe and severe saline soils have high pH value and salt content, and the herbaceous plants with strong resistance are mainly planted, and trees ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G17/00G01N21/31
CPCA01G17/005G01N21/3103
Inventor 李琳梁素钰田松岩杜倩刘铁男刘延坤王文帆王明强刘旭平
Owner HEILONGJIANG FOREST ENG & ENVIRONMENT INST