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A construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber

A construction method and technology of a simulation cabin are applied in the field of construction of a hypoxic hypoxia simulation cabin at high altitude to simulate the hypoxia model of rats, which can solve the problems of unclear pathogenesis of acute altitude sickness, and achieve a high production success rate and good stability. Effect

Inactive Publication Date: 2015-09-09
贾正平
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the pathogenesis of acute altitude sickness is still unclear, which is also an important reason for restricting the rational use of altitude sickness and the development of related drugs

Method used

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  • A construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber
  • A construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber
  • A construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber

Examples

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Effect test

Embodiment 1

[0071] Example 1 A method for constructing a plateau hypobaric hypoxia simulation cabin simulating a model of hypoxia in rats, comprising the following steps:

[0072] ⑴ Simulate an altitude of 3,500 meters in a high-altitude low-pressure hypoxic simulation cabin, and screen out hypoxia-sensitive rats through treadmill experiments on experimental rats.

[0073] Among them: experimental rats refer to SPF grade healthy Wistar and / or SD male rats, weighing 180g, aged 6-7 weeks.

[0074] The screening method refers to simulating an altitude of 3,500 meters in a plateau hypobaric and hypoxic simulation cabin, and 15 to 100 experimental rats are subjected to treadmill experiments at a speed of 10m / min and a slope of 5°C. The exercise time is used as the endurance evaluation standard, the 5 experimental rats with long exercise time are regarded as tolerance rats, the 5 experimental rats with middle exercise time are regarded as insensitive rats, and the 5 experimental rats with ...

Embodiment 2

[0081] Example 2 A method for constructing a plateau hypobaric hypoxia simulation cabin simulating a model of hypoxia in rats, comprising the following steps:

[0082] ⑴ Simulate an altitude of 3,500 meters in a high-altitude low-pressure hypoxic simulation cabin, and screen out hypoxia-sensitive rats through treadmill experiments on experimental rats.

[0083] Among them: the experimental rats refer to SPF grade healthy Wistar and / or SD male rats, weighing 300g, aged 6-7 weeks.

[0084]The screening method refers to simulating an altitude of 3,500 meters in a high-altitude low-pressure hypoxic simulation cabin, and 100 experimental rats are subjected to a treadmill experiment at a speed of 20m / min and a slope of 20°C to test the movement of the rats on the treadmill. Time is used as the endurance evaluation standard. The 40 experimental rats with long exercise time are regarded as tolerance rats, the 20 experimental rats with middle exercise time are regarded as insensit...

Embodiment 3

[0091] Example 3 A method for constructing a plateau hypobaric hypoxia simulation cabin simulating a model of hypoxia in rats, comprising the following steps:

[0092] ⑴ Simulate an altitude of 3,500 meters in a high-altitude low-pressure hypoxic simulation cabin, and screen out hypoxia-sensitive rats through treadmill experiments on experimental rats.

[0093] Among them: experimental rats refer to SPF grade healthy Wistar and / or SD male rats, weighing 200 g, aged 6-7 weeks.

[0094] The screening method refers to simulating an altitude of 3,500 meters in a high-altitude low-pressure hypoxic simulation cabin, and 30 experimental rats are subjected to a treadmill experiment at a speed of 15m / min and a slope of 15°C to test the movement of the rats on the treadmill. Time is used as the endurance evaluation standard. The 10 experimental rats with long exercise time are regarded as tolerance rats, the 10 experimental rats with middle exercise time are regarded as insensitive...

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Abstract

The invention provides a construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber. The method comprises the following steps: firstly, performing treadmill tests on experimental rats and screening out hypoxia-sensitive rats by simulating an environment with an altitude of 3500 meters in the highland low pressure hypoxia simulation chamber; secondly, selecting hypoxia-sensitive rats randomly and placing the selected rats in the highland low pressure hypoxia simulation chamber; thirdly establishing conditions for experimental rat hypoxia simulation of the highland low pressure hypoxia simulation chamber; fourthly, identifying the blood gas biochemical indexes and pathological indexes of the hypoxia-sensitive rats after hypoxia for 24-120h . The method is simple and easy to implement, is high in model making success rate, can truly reflect hypoxia injury conditions of animals and is high in stability.

Description

technical field [0001] The invention relates to the field of medical animal model research, in particular to a method for constructing a model of rat hypoxia simulated in a plateau hypobaric hypoxia simulation cabin. Background technique [0002] China has a vast plateau area, and more than 10 million people go to the plateau area from the inland every year for work and tourism. The harsh natural environment of the plateau seriously affects the health of people entering the plateau. According to statistics, 60-90% of the people in the plains quickly enter areas above 2500m above sea level and suffer from acute altitude sickness, which restricts the development of the national economy in plateau areas. [0003] Acute altitude sickness, that is, the body quickly enters the plateau area (the plateau in medicine refers to the area above 2500m above sea level, Qinghai standard) or enters a higher altitude area after living on the plateau for a long time, and the onset occurs with...

Claims

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Application Information

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IPC IPC(8): A01K1/03
Inventor 贾正平王荣李文斌谢华王昌鹿辉
Owner 贾正平
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