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siRNA inhibiting grb2 gene expression and application thereof

A gene expression and gene silencing technology, applied in the field of biomedicine, which can solve the problems of genotype transformation and drug resistance.

Inactive Publication Date: 2015-09-09
SHAOXING INST OF TECH COLLEGE OF ENG PEKING UNIV BIOENG CENT
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Problems solved by technology

Since EV71 virus tends to undergo genotype changes during transmission, it may develop drug resistance

Method used

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  • siRNA inhibiting grb2 gene expression and application thereof
  • siRNA inhibiting grb2 gene expression and application thereof
  • siRNA inhibiting grb2 gene expression and application thereof

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Embodiment Construction

[0019] Below in conjunction with specific accompanying drawing and embodiment the present invention will be further described

[0020] The present invention utilizes Northern blotting and Western blotting techniques to determine that RD cells can express Grb2 gene in large quantities at the RNA level, and EV71 can replicate in large quantities in RD cells, so the present invention adopts RD cells (from Institute of Cells, Chinese Academy of Sciences) as siRNA screening cells and EV71 invasion The cells were stained, and the cells were cultured in DMEM (Gibco, 12800-017) medium (containing 10% FBS) and the culture conditions were 37 degrees, 5% CO2.

[0021] 1. Design and synthesis of siRNA

[0022] According to the siRNA design principle, 5 pairs of siRNA were designed for the mRNA of the Grb2 gene (see Table 1). The design method and steps are: 1) use the nucleotide database of NCBI to find the RNA sequence of the human source Grb2 gene respectively, and its Accession in NCB...

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Abstract

Belonging to the technical field of biological medicine, the invention in particular relates to siRNA efficiently inhibiting growth factor receptor-bound protein2 (Grb2) gene expression and its application in anti-enterovirus71 (EV71) drugs. According to the design principle of siRNA, 5 pairs of siRNA directed at the Grb2 gene are designed, and the 5 pairs of siRNA can be used for inhibiting Grb2 gene expression. As the expression level of the Grb2 gene is closely related to replication of EV71, the siRNA inhibiting Grb2 gene expression can effectively delay or even block the pathological process of EV71, thereby providing feasible drugs for treatment of EV71.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an siRNA for efficiently inhibiting the expression of a growth factor receptor-bound protein 2 (Grb2) gene and an anti-enterovirus 71 (enterovirus71, EV71) drug thereof. aspects of application. Background technique [0002] Since HFMD was first reported in 1974, it caused outbreaks of varying degrees in Bulgaria, Hungary, Malaysia and Taiwan in 1975, 1978, 1997 and 1998, involving dozens of deaths. In March 2008, an outbreak of HFMD began in Fuyang, Anhui Province, my country, resulting in 23 deaths and more than 3,000 infections. After that, EV71 infection was also reported successively in Beijing, Shanghai, Zhejiang, Guangdong and other regions. By the end of 2008, the cumulative number of hand, foot and mouth disease infections in China had reached more than 489,000, and the death toll was 126. In 2009, after hand, foot and mouth disease infection was reported...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P31/14
Inventor 赵志敬沈文婷胡广
Owner SHAOXING INST OF TECH COLLEGE OF ENG PEKING UNIV BIOENG CENT
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