A kind of herbicide and its preparation method and application
A herbicide and herbicidal activity technology, applied in the field of pesticides, can solve problems such as the development gap of fungal herbicides, and achieve the effects of less usage, wide herbicidal spectrum and environmental safety
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[0033] The present invention provides a kind of preparation method of herbicide, it is characterized in that, comprises:
[0034] Step 1: Fermenting and culturing Trichoderma aeruginosa capable of producing herbicidally active substances to obtain a culture solution containing herbicidally active metabolites;
[0035] Step 2: performing a crude extraction operation on the obtained culture solution containing the herbicidally active metabolite to obtain a crude extract of the herbicidally active substance;
[0036]Step 3: using a common carrier for pesticides known in the art, formulating the crude extract into any dosage form to obtain the herbicide.
[0037] The Trichoderma aeruginosa is a strain preserved by the China General Microbiology Collection Center with the preservation number CGMCC No8722.
[0038] In the embodiment of the present invention, the step 1 adopts the conventional production method of microorganism-derived antibiotics, and culturing the Trichoderma auri...
Embodiment 1
[0054] Embodiment 1 - the preparation of the crude extract of herbicidal active substance
[0055] 3% glucose, 2.5% potato flour, 0.3% yeast powder, 0.1% yeast extract, 800.5% Tween, 0.05% potassium dihydrogen phosphate, 0.5% magnesium chloride, and 30.1% antifoaming agent LX-60 were prepared for shake flask culture base, adjust the pH to 7.0, shake the bottle with a volume of 150ml / 1L, and sterilize at 121°C for 20 minutes;
[0056] Trichoderm citrinoviridevs (Trichoderm citrinoviridevs) CGMCC No8722 bacterial classification that grows on the slant of potato sucrose medium is inoculated in this medium, and the shaker flask is placed at 28° C., 200 rpm, cultivated for 30-38 hours, and then with 5-15% The volume ratio of inoculated with the above medium 35L in a 50L fermenter, the aeration ratio is 1V / V, the tank pressure is 0.03Mpa, the temperature is 29°C, the stirring speed is 200rpm, and the culture is 92-96 hours. After the fermentation is completed, the herbicidal active ...
Embodiment 2
[0060] Embodiment 2——preparation of wettable powder
[0061] Get 30 parts of the crude extract obtained in Example 1 (weight ratio, the same below), 6 parts of Borresperse NA, 122 parts of K, 4 parts of white carbon black, 58 parts of kaolin, mix, and jet mill to obtain a wettable powder with a content of 30%.
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