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A kind of herbicide and its preparation method and application

A herbicide and herbicidal activity technology, applied in the field of pesticides, can solve problems such as the development gap of fungal herbicides, and achieve the effects of less usage, wide herbicidal spectrum and environmental safety

Active Publication Date: 2018-07-10
SHANGHAI WANLIHUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the research direction of microbial herbicides in China is mostly concentrated in the field of actinomycetes and bacteria, and the development of fungal herbicides is still blank.

Method used

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  • A kind of herbicide and its preparation method and application
  • A kind of herbicide and its preparation method and application
  • A kind of herbicide and its preparation method and application

Examples

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preparation example Construction

[0033] The present invention provides a kind of preparation method of herbicide, it is characterized in that, comprises:

[0034] Step 1: Fermenting and culturing Trichoderma aeruginosa capable of producing herbicidally active substances to obtain a culture solution containing herbicidally active metabolites;

[0035] Step 2: performing a crude extraction operation on the obtained culture solution containing the herbicidally active metabolite to obtain a crude extract of the herbicidally active substance;

[0036]Step 3: using a common carrier for pesticides known in the art, formulating the crude extract into any dosage form to obtain the herbicide.

[0037] The Trichoderma aeruginosa is a strain preserved by the China General Microbiology Collection Center with the preservation number CGMCC No8722.

[0038] In the embodiment of the present invention, the step 1 adopts the conventional production method of microorganism-derived antibiotics, and culturing the Trichoderma auri...

Embodiment 1

[0054] Embodiment 1 - the preparation of the crude extract of herbicidal active substance

[0055] 3% glucose, 2.5% potato flour, 0.3% yeast powder, 0.1% yeast extract, 800.5% Tween, 0.05% potassium dihydrogen phosphate, 0.5% magnesium chloride, and 30.1% antifoaming agent LX-60 were prepared for shake flask culture base, adjust the pH to 7.0, shake the bottle with a volume of 150ml / 1L, and sterilize at 121°C for 20 minutes;

[0056] Trichoderm citrinoviridevs (Trichoderm citrinoviridevs) CGMCC No8722 bacterial classification that grows on the slant of potato sucrose medium is inoculated in this medium, and the shaker flask is placed at 28° C., 200 rpm, cultivated for 30-38 hours, and then with 5-15% The volume ratio of inoculated with the above medium 35L in a 50L fermenter, the aeration ratio is 1V / V, the tank pressure is 0.03Mpa, the temperature is 29°C, the stirring speed is 200rpm, and the culture is 92-96 hours. After the fermentation is completed, the herbicidal active ...

Embodiment 2

[0060] Embodiment 2——preparation of wettable powder

[0061] Get 30 parts of the crude extract obtained in Example 1 (weight ratio, the same below), 6 parts of Borresperse NA, 122 parts of K, 4 parts of white carbon black, 58 parts of kaolin, mix, and jet mill to obtain a wettable powder with a content of 30%.

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Abstract

The invention provides a method for preparing a herbicide, comprising: step 1: fermenting and culturing Trichoderma aurantiolis capable of producing herbicidally active substances to obtain a culture solution containing herbicidally active metabolites; step 2: preparing the obtained herbicidally active metabolites The culture solution of active metabolites is subjected to a crude extraction operation to obtain a crude extract of herbicidal active substances; step 3: using a carrier commonly used in pesticides known in the art, the crude extract is formulated into any dosage form to obtain the herbicide. The herbicide is a new type of herbicide with crude metabolites of Trichoderma auranthoderma as an active ingredient. It has the characteristics of less usage, wide herbicidal spectrum, and environmental arrangement, and can be widely used in paddy fields and dry fields. sexual weeding.

Description

technical field [0001] The invention relates to the field of pesticides, in particular to a herbicide, in particular to a herbicide with microbial metabolites as active ingredients, a preparation method and application thereof. Background technique [0002] With the widespread use of chemical herbicides in our country, the negative impact on the environment has become increasingly significant. Extensive use of chemical pesticides not only pollutes groundwater, but also causes land degradation. From a long-term point of view, the residual toxicity and phytotoxicity caused by the extensive use of long-residual herbicides may even lead to severe crop yield reduction. At present, more than 100 kinds of chemical herbicides have been banned in more than 30 countries in the world. But this move has brought about a new problem, that is, repeated application of a single species of herbicide has led to the formation of resistant weed populations. According to the International Herb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P13/00
Inventor 旷文丰陈娟王承芳陈晨干华磊毛伟力
Owner SHANGHAI WANLIHUA BIOTECH