A kind of fermentation method of fermented liquid feed
A fermentation method and liquid feed technology, applied in animal feed, animal feed, animal husbandry, etc., can solve problems such as insoluble nutrition, inhomogeneity, and reduced equipment utilization, and achieve enhanced fermentation mass transfer and oxygen energy process. The effect of uniform and stable quality and reduced energy consumption for fermentation and stirring
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Embodiment 1
[0027] As an embodiment of the present invention, the fermentation method of described fermented liquid feed comprises the following steps:
[0028] (1) Obtaining of fermentation strains:
[0029] Saccharomyces cerevisiae was transferred to the slant of YPD medium, and cultured at 28-36° C. for 16-26 hours.
[0030] In this example, the Saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, which is deposited in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.6120.
[0031] Inoculate the obtained slant of Saccharomyces cerevisiae into liquid medium, culture it on a shaker at 28-36°C for 12-16 hours, and obtain the first-grade Saccharomyces cerevisiae seed liquid; take the first-grade Saccharomyces cerevisiae seed liquid, and inoculate in the In the secondary seed culture medium, cultivate under the same conditions to obtain the secondary Saccharomyces cerevisiae seed liquid.
[0032] (2) Fermentation pretreat...
Embodiment 2
[0042] As an embodiment of the present invention, the fermentation method of described fermented liquid feed comprises the following steps:
[0043] (1) Obtaining of fermentation strains:
[0044] Saccharomyces cerevisiae was transferred to the slant of YPD medium, and cultured at 30-36° C. for 16-24 hours.
[0045] In this example, the Saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, which is deposited in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.6120.
[0046] Inoculate the obtained Saccharomyces cerevisiae slant into liquid medium, and culture it on a shaker at 28-36°C for 12-15 hours to obtain the first-grade Saccharomyces cerevisiae seed liquid; take the first-grade Saccharomyces cerevisiae seed liquid and inoculate it in the In the secondary seed medium, cultivate under the same conditions to obtain the secondary Saccharomyces cerevisiae seed liquid.
[0047] (2) Fermentation pretreatment
...
Embodiment 3
[0058] As an embodiment of the present invention, the fermentation method of described fermented liquid feed comprises the following steps:
[0059] (1) Obtaining of fermentation strains:
[0060] Saccharomyces cerevisiae was transferred to the slant of YPD medium, and cultured at 30-36° C. for 16-24 hours.
[0061] In this example, the Saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, which is deposited in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.6120.
[0062] Inoculate the obtained Saccharomyces cerevisiae slant into liquid medium, and culture it on a shaker at 28-36°C for 12-15 hours to obtain the first-grade Saccharomyces cerevisiae seed liquid; take the first-grade Saccharomyces cerevisiae seed liquid and inoculate it in the In the secondary seed medium, cultivate under the same conditions to obtain the secondary Saccharomyces cerevisiae seed liquid.
[0063] (2) Fermentation pretreatment
...
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