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A kind of fermentation method of fermented liquid feed

A fermentation method and liquid feed technology, applied in animal feed, animal feed, animal husbandry, etc., can solve problems such as insoluble nutrition, inhomogeneity, and reduced equipment utilization, and achieve enhanced fermentation mass transfer and oxygen energy process. The effect of uniform and stable quality and reduced energy consumption for fermentation and stirring

Active Publication Date: 2018-10-19
北京英惠尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this case, due to the increase in viscosity, the energy consumption such as stirring will increase significantly, and the energy consumption in the fermentation process accounts for a very large part of the fermentation cost, and the increase in energy consumption will greatly increase the production cost; in addition, due to the increase in material viscosity , the process of oxygen and mass transfer is blocked, the fermentation process is forced to prolong, and the utilization rate of the equipment is reduced. At the same time, due to the poor fluidity of the material, the product quality will be unstable and uneven, which will affect the promotion and application of the product; the material has a high viscosity. At the same time, it also causes serious sticky walls, which brings great difficulties to the cleaning of the fermentation system and feeding system, and the residual materials will cause the growth of spoilage bacteria, especially in breeding enterprises due to the cumbersome work, which greatly affects the Approval and promotion of related feed
[0007] Third, there is usually no step of high-temperature treatment to remove anti-nutritional factors in the fermentation feed liquid. Simply relying on the fermentation process, a large amount of anti-nutritional factors remain in the fermented material
For example, phytic acid in cereals can strongly bind to metal ions such as Ca, Fe, Mg, Zn and Mn, making them insoluble and thus making nutrients unavailable (Bohn L, Journal of Zhejiang University Science B9, 165-191 (2008) )
However, the high-temperature aging will cause the material to agglomerate or become a complete solid state, so that subsequent fermentation cannot be carried out.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] As an embodiment of the present invention, the fermentation method of described fermented liquid feed comprises the following steps:

[0028] (1) Obtaining of fermentation strains:

[0029] Saccharomyces cerevisiae was transferred to the slant of YPD medium, and cultured at 28-36° C. for 16-26 hours.

[0030] In this example, the Saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, which is deposited in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.6120.

[0031] Inoculate the obtained slant of Saccharomyces cerevisiae into liquid medium, culture it on a shaker at 28-36°C for 12-16 hours, and obtain the first-grade Saccharomyces cerevisiae seed liquid; take the first-grade Saccharomyces cerevisiae seed liquid, and inoculate in the In the secondary seed culture medium, cultivate under the same conditions to obtain the secondary Saccharomyces cerevisiae seed liquid.

[0032] (2) Fermentation pretreat...

Embodiment 2

[0042] As an embodiment of the present invention, the fermentation method of described fermented liquid feed comprises the following steps:

[0043] (1) Obtaining of fermentation strains:

[0044] Saccharomyces cerevisiae was transferred to the slant of YPD medium, and cultured at 30-36° C. for 16-24 hours.

[0045] In this example, the Saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, which is deposited in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.6120.

[0046] Inoculate the obtained Saccharomyces cerevisiae slant into liquid medium, and culture it on a shaker at 28-36°C for 12-15 hours to obtain the first-grade Saccharomyces cerevisiae seed liquid; take the first-grade Saccharomyces cerevisiae seed liquid and inoculate it in the In the secondary seed medium, cultivate under the same conditions to obtain the secondary Saccharomyces cerevisiae seed liquid.

[0047] (2) Fermentation pretreatment

...

Embodiment 3

[0058] As an embodiment of the present invention, the fermentation method of described fermented liquid feed comprises the following steps:

[0059] (1) Obtaining of fermentation strains:

[0060] Saccharomyces cerevisiae was transferred to the slant of YPD medium, and cultured at 30-36° C. for 16-24 hours.

[0061] In this example, the Saccharomyces cerevisiae is Saccharomyces cerevisiae Sa-10, which is deposited in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.6120.

[0062] Inoculate the obtained Saccharomyces cerevisiae slant into liquid medium, and culture it on a shaker at 28-36°C for 12-15 hours to obtain the first-grade Saccharomyces cerevisiae seed liquid; take the first-grade Saccharomyces cerevisiae seed liquid and inoculate it in the In the secondary seed medium, cultivate under the same conditions to obtain the secondary Saccharomyces cerevisiae seed liquid.

[0063] (2) Fermentation pretreatment

...

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PUM

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Abstract

The invention discloses a fermentation method for fermenting liquid fodder. The method comprises the following steps that water is added into cereal raw materials, even mixing is carried out, mixture liquid is prepared, and the pH value of the mixture liquid is regulated to range from 5.5 to 6.5; amylase is added into the mixture liquid, thermal insulating processing is carried out; high-temperature sterilizing is carried out on the thermal insulated mixture liquid in advance, cooling is carried out, and fermenting bacteria are inoculated into the mixture liquid to be subjected to fermenting. According to the fermenting method, the problem that the product viscosity is greatly improved after sterilizing is carried out is solved, the material viscosity can be reduced for 20 times to 7000 times or more, the energy consumption of fermenting and stirring can be greatly lowered, and the fermenting cost is lowered. In addition, due to the fact that the viscosity is lowered, the material fluidity is obviously improved, and the fermenting mass and oxygen transferring processed can be obviously enhanced, so that the fermenting process is accelerated, and the product quality is evener and more stable.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to a fermentation method for fermenting liquid feed. Background technique [0002] In recent years, the development of my country's animal husbandry and breeding industry has advanced by leaps and bounds, and the breeding industry has continued to develop in a large-scale and integrated manner. However, the abuse of drugs such as antibiotics and environmental pollution that emerged during the development process have attracted more and more attention. Today, when food safety is of great concern, many countries have banned the use of antibiotics in feed. The future green ecological breeding method is an inevitable development direction. Fermented liquid feed is a hotspot in research at home and abroad in terms of healthy farming. Numerous experimental data show that this new type of feed has many incomparable advantages over traditional feed. Fermented liquid feed has the characteris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12
Inventor 王宏郭庆李慧张虎江衍哲余际国黄吉波李守勇
Owner 北京英惠尔生物技术有限公司
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