A method for detecting vascular endothelial growth factor based on tetrahedral silver embedded structure
A vascular endothelium and growth factor technology, applied in the field of analytical chemistry, can solve the problems of high monoclonal antibody, high consumption, heavy screening of monoclonal antibody, etc., and achieve the effect of low detection limit and high sensitivity
Active Publication Date: 2017-07-07
JIANGNAN UNIV
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Problems solved by technology
[0003] Traditional VEGF detection methods are mainly enzyme-linked immunoassay, etc., which have high requirements for monoclonal antibodies, and the screening of monoclonal antibodies is a heavy and costly task
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Embodiment 1
[0038] (1) Construction of the detection sensor
[0039] (1) Synthesis of gold nanoparticles with particle size of 10nm
[0040] Gold nanoparticles with a particle size of 10nm were synthesized by the tannic acid reduction method. The Erlenmeyer flask used for the reaction was soaked in newly prepared aqua regia for 24 hours in advance, rinsed with ultrapure water until clean, and dried for later use. Add 79mL ultrapure water and 1mL chloroauric acid with a mass concentration of 1% in the Erlenmeyer flask as solution A; take another clean small bottle, add 4 mL of 1% trisodium citrate, 0.1mL of 1% tannic acid , 0.1mL 25mM potassium carbonate, 15.8mL ultrapure water as B solution. Both liquids A and B were heated to 60°C, then quickly added liquid B to liquid A under high-speed stirring, and the mixture continued to stir at 60°C for 30 minutes until a deep red solution was formed. The solution was then heated to reflux for 2 min to form a bright red solution. Finally, it was...
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Abstract
The invention relates to an ultrasensitive method for detecting vascular endothelial growth factor based on a silver-embedded tetrahedron structure, which belongs to the field of analytical chemistry. In the present invention, the synthesis of gold nanoparticles with a particle size of 10nm, the synthesis of silver nanoparticles with a particle size of 6nm, the encapsulation of gold nanoparticles, the modification of single-stranded DNA on gold nanoparticles, and the aptamer of vascular endothelial growth factor on silver nanoparticles Modification of Apt-VEGF and beacon molecule 4-NTP, assembly of silver-embedded tetrahedral structure and detection of surface-enhanced Raman scattering signal intensity, and establishment of a standard curve of Raman signal intensity and vascular endothelial growth factor concentration. The invention provides a method for detecting vascular endothelial growth factor based on a silver-embedded tetrahedron structure, which is low in cost, high in sensitivity, convenient and quick compared with traditional detection methods, and has good practical application prospects.
Description
technical field [0001] The invention relates to a method for ultrasensitive detection of vascular endothelial growth factor based on a tetrahedral silver-embedded structure, belonging to the field of analytical chemistry. Background technique [0002] Vascular endothelial growth factor (VEGF) is a type of glycoprotein that acts on vascular endothelial cells, exists in the form of homodimers, has a strong effect on promoting endothelial cell proliferation, and promotes the formation of new blood vessels. The growth of tumor tissue must rely on angiogenesis to provide sufficient oxygen and nutrients for maintenance. Therefore, VEGF has been considered as a tumor marker. The detection of tumor markers helps to detect cancer early and achieve the purpose of early treatment. [0003] Traditional VEGF detection methods are mainly enzyme-linked immunoassay, etc., which have high requirements for monoclonal antibodies, and the screening of monoclonal antibodies is a heavy and costl...
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IPC IPC(8): G01N21/65C12Q1/68
Inventor 胥传来赵森匡华徐丽广马伟刘丽强宋珊珊胡拥明
Owner JIANGNAN UNIV