Traditional Chinese medicine compound composition for treating piglet pullorum
A composition and technology of traditional Chinese medicine, applied in the field of compound Chinese medicine composition, can solve the problems of reduced therapeutic effect and low drug resistance
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Embodiment 1
[0014] 1. Experiment on the treatment of diarrhea in mice
[0015] 1.1 Experimental animals and strains
[0016] 20±2g male Kunming mice were purchased from Shanghai Slack Experimental Animal Co., Ltd.; pathogenic porcine Escherichia coli were purchased from China National Institute of Pharmaceutical and Biological Products.
[0017] 1.2 Drug preparation
[0018] 50 grams of Coptis chinensis, 45 grams of Scutellaria baicalensis, 45 grams of Pulsatilla chinensis, 45 grams of Qinpi, 45 grams of dandelion, 45 grams of kudzu root, 40 grams of gallnut, 40 grams of Cortex Phellodendri, 40 grams of raw rhubarb, 30 grams of forsythia, 40 grams of honeysuckle, 40 grams of astragalus and Distilled water was mixed according to the weight ratio of 1:10, soaked for 30 minutes, decocted twice for 30 minutes each time, combined the filtrate, then filtered to remove impurities, and concentrated under reduced pressure to obtain Chinese medicine compound water containing crude drug 1g / mL for t...
Embodiment 2
[0034] 2. Treatment of piglet diarrhea test
[0035] 2.1 Test drugs
[0036] Chinese medicine compound decoction is the same as in Example 1. Bacterial classification is with embodiment 1.
[0037] 2.2 Test animals
[0038] Thirty 21-day-old Du and Sanyuan hybrid piglets.
[0039] 2.3 Experimental design
[0040] Thirty 21-day-old piglets were randomly divided into 3 groups, namely compound drug group, blank control group and model control group, with 10 piglets in each group. The compound drug group and the model control group were fed with 5mL Escherichia coli liquid (3×10 9 CFU / mL) for 3 consecutive days; the blank control group was fed with 5 mL of normal saline every day for 3 consecutive days. The compound medicine group was given compound decoction 10mL / 10Kg per head, twice a day, for 7 consecutive days; the blank control group and the model control group were given the same dose of normal saline. The piglets are raised at an ambient temperature of about 25°C, fr...
Embodiment 3
[0054] 3. Experiment on the treatment of pullorum in piglets
[0055] 3.1 Test drugs
[0056] Chinese medicine compound decoction is the same as in Example 1. Shigella pure powder, batch number 20140707, specification 100g / bag.
[0057] 3.2 Test animals
[0058] Select 100 weaned piglets suffering from pullorum in the pig herd.
[0059] 3.3 Experimental design
[0060] 100 sick weaned piglets were divided into two groups, 50 pigs in each group, and the compound Chinese medicine composition and dysentery pure powder were used respectively. The Chinese medicine compound composition solution was taken orally, twice a day for each head, 5ml each time; the dysentery pure powder was administered in drinking water, and 1 gram of medicine was added to every kilogram of water.
[0061] 3.4 Measuring indicators
[0062] The cure rate is the percentage of the number of cured heads to the number of treated heads. The number of cured cases refers to the disappearance or reduction of...
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