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Application of high-strength light sensitive hydrogel in action of adjusting cell attachment

A high-intensity light and hydrogel technology, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., can solve problems such as gel damage and weak mechanical properties of hydrogel, and achieve high water absorption and significant light Sensitivity, effect of simple preparation method

Inactive Publication Date: 2015-10-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for traditional hydrogels, the dilution effect of water molecules in the gel on the polymer network, the disordered distribution of cross-linking points and the length of chain links, and the inhomogeneity of the cross-linking network during the gelation process cause The mechanical properties of hydrogel are very weak, it is difficult to be used as a soft tissue replacement under high load, and even the touch of tweezers and other utensils during the movement can easily damage the gel, thus limiting its application as a biomaterial

Method used

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  • Application of high-strength light sensitive hydrogel in action of adjusting cell attachment
  • Application of high-strength light sensitive hydrogel in action of adjusting cell attachment
  • Application of high-strength light sensitive hydrogel in action of adjusting cell attachment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The monomer oligoethylene glycol methacrylate (6mg), 2-vinyl-4,6-diamino-1,3,5-triazine (6mg), SP (0.6mg) and crosslinking agent Ethylene glycol diacrylate (8.4mg) was added to a 1.5ml centrifuge tube, 210μl of DMSO was used to dissolve the monomer and crosslinker, and then the photoinitiator Irgacure 2959 (0.5mg) was added. The solvent containing monomer, crosslinking agent and initiator is injected into a closed mold, and the mold is irradiated in a UV curing box for 30 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water and soaked for 7 days, and the deionized water was replaced every 12h.

[0033] Cell culture and desorption on the surface of the gel: The obtained gel was immersed in medical alcohol for sterilization for 4 hours, then placed in a 96-well plate, and 200μl of DMEM medium was added to replace the medical alcohol. Add L929 cell suspension to a culture plate with gel and i...

Embodiment 2

[0036] The monomer oligoethylene glycol methacrylate (6mg), 2-vinyl-4,6-diamino-1,3,5-triazine (12mg), SP (0.9mg) and crosslinking agent Ethylene glycol diacrylate (12.6mg) was added to a 1.5ml centrifuge tube. After dissolving the monomer and crosslinker with 315μl of DMSO, the photoinitiator Irgacure 2959 (0.7mg) was added. The solvent containing monomers, crosslinking agent and initiator is injected into a closed mold, and the mold is irradiated in an ultraviolet curing box for 30 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water and soaked for 7 days, and the deionized water was replaced every 12h.

[0037] Cell culture and desorption on the surface of the gel: The obtained gel was immersed in medical alcohol for sterilization for 4 hours, then placed in a 96-well plate, and 200μl of DMEM medium was added to replace the medical alcohol. Add L929 cell suspension to a culture plate with gel ...

Embodiment 3

[0040] The monomer oligoethylene glycol methacrylate (6mg), 2-vinyl-4,6-diamino-1,3,5-triazine (18mg), SP (1.2mg) and crosslinking agent Ethylene glycol diacrylate (16.8mg) was added to a 1.5ml centrifuge tube. After dissolving the monomer and crosslinker with 420μl of DMSO, the photoinitiator Irgacure 2959 (0.9mg) was added. The solvent containing monomer, crosslinking agent and initiator is injected into a closed mold, and the mold is irradiated in a UV curing box for 30 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water and soaked for 7 days, and the deionized water was replaced every 12h.

[0041] Cell culture and desorption on the surface of the gel: The obtained gel was immersed in medical alcohol for sterilization for 4 hours, then placed in a 96-well plate, and 200μl of DMEM medium was added to replace the medical alcohol. Add L929 cell suspension to a culture plate with gel and incubat...

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Abstract

The invention discloses high-strength photosensitive hydrogel as well as a preparation method and application thereof. The high-strength photosensitive hydrogel is formed by free radical polymerization copolymerization of polyethylene glycol methacrylic ester, 2-vinyl-4,6-diamino-1,3,5-triazine and a double-bond-containing monomer with a spiropyrane structure; the three monomers and an initiator are dissolved in a solvent, unsaturated bonds on the molecules of the monomers are initiated by the initiator, and the hydrogel is prepared through free radical polymerization reaction. The photosensitive hydrogel disclosed by the invention has higher stretching resistance and compression resistance, and has good biocompatibility. Moreover, the hydrogel has the capability of adsorbing DNA (Deoxyribonucleic Acid) transfection cells as well as adjusting attaching and un-attaching behaviors of the cells by illumination.

Description

[0001] The application of the present invention is a divisional application of the parent application "High-intensity light-sensitive hydrogel and its preparation method and application", the parent application number is 201310473943.1, and the filing date is October 11, 2013. Technical field [0002] The technical field of polymer hydrogels of the present invention, more specifically, relates to the preparation of high-strength hydrogels with the ability to detach cells and transgenic effects, namely, polyethylene glycol methacrylate-copolymer-2-vinyl- The preparation of 4,6-diamino-1,3,5-triazine-copolymer-containing spiropyran monomer copolymer (PEGMA-co-PVDT-co-PSP) hydrogel and its application. Background technique [0003] Polymer hydrogel is a kind of three-dimensional network polymer with high water content and can maintain the original structure without being dissolved after absorbing water. Because of its characteristics similar to soft tissue and good biocompatibility, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F290/06C08F226/06C08F220/60C08F2/48C12N5/09C12N15/85
Inventor 刘文广王宁张金龙孙亮王朋延
Owner TIANJIN UNIV
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