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Polyphagous sericin silkworm strain and its establishment method

A method for establishing a silkworm and a technology for silkworm, which are applied in the field of wide-eating sericin silkworm strains and their establishment, and can solve the problems of low mulberry leaf content and poor food intake

Inactive Publication Date: 2018-08-24
国立研究开发法人农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, all of the above sericin silkworm strains have a big problem of being poor in feeding properties of artificial diets with a low mulberry leaf content (Non-Patent Documents 5 and 6)

Method used

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  • Polyphagous sericin silkworm strain and its establishment method
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  • Polyphagous sericin silkworm strain and its establishment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] (Purpose)

[0107] The changes in the feeding rate, cocooning rate and pupation rate of the artificial diet with low mulberry leaf content in the polyphagous sericin silkworm strain individuals obtained by mating the cocooning sericin silkworm strain and the polyphagous silkworm strain were verified.

[0108] (method)

[0109] 1. Mating

[0110] The cocooning sericin silkworm strain Sericin-hope (Japanese Patent No. 3374177) and the polyphagous multifilament silkworm strain (TN38, TCS40) were mated. Specifically, the female Sericin-hope was mated with the male polyphagous silkworm silkworm strain. Afterwards, intra-line mating was carried out for each hybrid individual, and the mating was repeated until the sixth generation (F6) of the hybrid.

[0111] 2. Incubation and transfer of eggs and rearing

[0112] The incubation and transfer of eggs is to mix the eggs laid by 4 to 10 females of the same line to hatch them. The hatched larvae are moved from the egg cardboa...

Embodiment 2

[0120] (Purpose)

[0121] The method for establishing a polyphagous sericin silkworm strain of the present invention is used to establish a cocoon-forming polyphagous sericin silkworm strain (MNS300 silkworm strain).

[0122] (method)

[0123] 1. Breeding

[0124] The flow chart of breeding is shown in Figure 4 . First, the female cocooning sericin silkworm strain Sericin-hope was mated with the male polyphagous multi-filament strain TN38, and the hatched larvae were reared with SilkMate L4M (Nippon Agricultural Industries) to select polyphagy ( first mating procedure). Simultaneously, among the individuals who cultivate cocoon silk gum cocoons, select individuals with more cocoon layer weight (only the weight of the cocoon: equivalent to the amount of silk spinning / only). Then, intra-line mating was performed for two generations, and TN38 was backcrossed twice (second mating step), and then intra-line mating was performed once. The artificial feed feeding ability of th...

Embodiment 3

[0129] (Purpose)

[0130] A cocooning polyphagous sericin silkworm strain (MCS300 silkworm strain) was established by using the method for establishing a polyphagous sericin silkworm strain of the present invention in a process different from that in Example 2.

[0131] (method)

[0132] 1. Breeding

[0133] The flow chart of breeding is shown in Figure 5 . First, the female Sericin-hope was mated with the male polyphagous multifilament strain TCS40 (the first mating process). The obtained hybrid individuals were mated once within the strain (selection step of polyphagous sericin silkworm), backcrossed with TCS40 (second mating step), and then mated with Sericin-hope (cocooning mating step). Then, backcross TCS40 again (the second mating procedure), and perform intra-line mating once (the selection procedure of cocooning polyphagous sericin silkworm). Next, the hybrid individual was mated with a cocoon-forming polyphagous F1 individual obtained by mating Zhong 604 and Se...

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Abstract

The present invention addresses the problem of providing a method and the like for producing a euryphagous sericin silkworm strain that can also be raised throughout the entire larval stage thereof on low-cost artificial feed containing almost no mulberry leaf. Provided is a method of producing a euryphagous sericin silkworm strain that includes crossbreeding a euryphagous silkworm strain with a sericin silkworm strain having a mutation in a gene related to a synthesis pathway for fibroin and crossbreeding the obtained hybrid specimen at least twice with a euryphagous silkworm strain, then performing breeding within the resulting strain at least five times on the basis of the traits of the sericin silkworm strain and the traits of the euryphagous silkworm strain.

Description

technical field [0001] The invention relates to a polyphagous sericin silkworm strain and a method for establishing the same. The polyphagous sericin silkworm strain can be fed at the whole larval stage even with artificial feed with a low mulberry leaf content. Background technique [0002] Many silkworms (Bombyx mori) are easy to rear and can synthesize a large amount of protein in a short period of time compared with bacteria such as Escherichia coli, so they are useful organisms as hosts for producing recombinant proteins and the like. In the case of producing a target recombinant protein using silkworms, a method of synthesizing it in silk glands producing silk is known (Non-Patent Documents 1-3). The silk gland is easy to remove due to its large size, and the synthesized protein is stored in the lumen of the middle silk gland, so it is also suitable for recovering the target recombinant protein. However, a large amount of fibroin, which is a fibrous protein constituti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01K67/04C07K14/435C12P21/02
CPCA01K67/04A01K2227/706A01K2267/02C12P21/02
Inventor 饭塚哲也冈田英二立松谦一郎
Owner 国立研究开发法人农业生物资源研究所