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A rubber tree flowering regulatory protein hbtfl1-2 and its coding gene and application

A technology for flowering regulation and coding genes, which can be applied in the fields of application, genetic engineering, and plant gene improvement, and can solve the problems that have not yet been reported on TFL1/CEN-like genes

Active Publication Date: 2018-05-15
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about TFL1 / CEN-like gene in Hevea brasiliensis

Method used

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  • A rubber tree flowering regulatory protein hbtfl1-2 and its coding gene and application
  • A rubber tree flowering regulatory protein hbtfl1-2 and its coding gene and application
  • A rubber tree flowering regulatory protein hbtfl1-2 and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Obtaining of 5 target genes of Hevea brasiliensis

[0032] According to the Rubber Tree Genome Database and Leaf Transcriptome Database of the Rubber Research Institute of the Chinese Academy of Tropical Agricultural Sciences, contigs obtained through homologous alignment are then spliced, and then the open reads are predicted by NCBI online software and specific primers are designed to obtain contigs containing complete open reads. DNA sequence and cDNA sequence of the reading frame.

[0033] The specific method is as follows:

[0034] Acquisition of open reading frames for 5 genes

[0035] The five gene-specific primers are as follows:

[0036] HbTFL1-1OF (EcoRI):G ATGTCAAGGATCATGGAGCC

[0037] HbTFL1-1OR(XbaI):GC TCATTCTTCTTCTTGCAGCAGTTTT

[0038] HbTFL1-2OF(EcoRI):G ATGGCAAGAATAATAGAACCTCT

[0039] HbTFL1-2OR(XbaI):GC TTAGCGCCTTCTTGCAGCAGTT

[0040] HbTFL1-3OF(EcoRI):G ATGGCAAGAATAATAGAACCTCT

[0041] HbTFL1-3OR(XbaI):GC TTAGCGTCTTCTT...

Embodiment 2

[0101] Example 2 Analysis of tissue-specific expression of 5 target genes

[0102] In this study, 3-month-old rubber tissue culture seedlings were selected as experimental materials. The roots, stems, buds, bronze leaves, discolored leaves, light green leaves and stable leaves were collected respectively, and their RNAs were extracted for future use.

[0103] The RNA obtained above was digested with DnaseI and then reverse transcribed according to the reverse transcription kit. The tissue-specific expression analysis of five target genes was performed by fluorescence quantitative technology.

[0104] The five gene fluorescent quantitative primers are:

[0105] HbTFL1-1QF:CCTCCTTAATCCCTGGCTACG,

[0106] HbTFL1-1QR:ACATCGCCTACAACTCTCCCT,

[0107] HbTFL1-2QF:GCCACATTTGGAAGGGAGATAG,

[0108] HbTFL1-2QR: TAACACTTATTTATTGAAGGCCACTTG,

[0109] HbTFL1-3QF: ATACACCTCTTAGCCAGACGCTC,

[0110] HbTFL1-3QR:TGTCGCCTCCTTGGACCTCA,

[0111] HbCEN1QF:CTAATGGCGAAGACAACAGAT,

[0112] HbCE...

Embodiment 3

[0116] Example 3 Analysis of spatiotemporal expression patterns of 5 target genes

[0117] Taking 3 months, 2 years and 10 years as the research objects, the roots, stems, buds, bronze leaves, color-changing leaves, light green leaves and stable leaves of each age group were collected in the flowering season (March) respectively, and 10 years old. RNA was extracted from the male and female flowers of the annual tree when they bloomed.

[0118] The RNA obtained above was digested with DnaseI and then reverse transcribed according to the reverse transcription kit. The temporal and spatial expression patterns of the five target genes were analyzed by fluorescence quantitative technology.

[0119] The results showed that: in the flowering season, for TFL1-like genes, the expressions of HbTFL1-1, HbTFL1-2 and HbTFL1-33 genes in their respective vegetative organs in 10-year-old trees were significantly lower than those in 3-month-old and 2-year-old trees . In the newly differenti...

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Abstract

The invention provides a rubber tree blooming regulation protein HbTFL1-2, an encoding gene thereof, and an application of the gene. The amino acid sequence of the blooming regulation protein HbTFL1-2 is represented by SEQ ID No.6, and the encoding gene of the protein is used to transform plants in order to obtain transgenic plants with delayed blooming stage.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rubber tree flowering regulation protein HbTFL1-2 and its encoding gene and application. Background technique [0002] TFL1 / CEN-like genes widely exist in flowering plants and are a very important flowering suppressor. They achieve the purpose of inhibiting flowering by inhibiting the downstream floral organ determination genes AP1 and LFY genes of the FT gene. [0003] In Arabidopsis tfl1 mutants, the vegetative phase of Arabidopsis is significantly shortened, concomitant with the transition of the inflorescence from an infinity to a terminal inflorescence. Therefore, it is proved that Arabidopsis TFL1 gene has the function of simultaneously regulating vegetative growth and inflorescence development (BradleyD, Ratcliffe O, Vincent C, Carpenter R, Coen E (1997) Inflorescence commitment and architecture in Arabidopsis. Science 275:80-83). In pea, two TFL1-like genes were shown to ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N1/21A01H5/02
CPCC07K14/415C12N15/827
Inventor 华玉伟黄华孙毕政鸿黄天带应佳志陈涛
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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