Haemophilus strain preservation method
A technology for preservation of Haemophilus and strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, preservation of microorganisms, etc., can solve the cumbersome preservation of Haemophilus, the inability to effectively cultivate Haemophilus, and the survival of Haemophilus Short term and other issues, to achieve the effect of stable biochemical properties, low cost, and long storage time
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Embodiment 1
[0014] This Haemophilus strain preservation method includes the following steps:
[0015] The first step is to make the culture medium. Mix 100ml of glycerol and 100ml of distilled water to make the primary culture medium. Then put the primary culture medium into a sterilizer for sterilization. The sterilization temperature is 121°C and the sterilization time is 15 minutes. After the pressure of the pressure gauge of the sterilizer drops to zero, immediately remove the primary medium. At this time, the temperature of the primary medium is 95°C, and then slowly add 25ml of sterile defiberized sheep blood to the primary medium to obtain glycerol Lamb's blood chocolate soup, after the glycerin lamb's blood chocolate soup is cooled to room temperature, dispense it into 10ml sterile test tubes, and each test tube contains 5ml glycerin lamb's blood chocolate soup to obtain a chocolate medium;
[0016] In the second step, the Haemophilus strains are stored, and the Haemophilus standard st...
Embodiment 2
[0020] This Haemophilus strain preservation method includes the following steps:
[0021] The first step is to make the culture medium. Mix 100ml of glycerol and 100ml of distilled water to make the primary culture medium. Then put the primary culture medium into a sterilizer for sterilization. The sterilization temperature is 121°C and the sterilization time is 15 minutes. After the pressure of the pressure gauge of the sterilizer drops to zero, immediately remove the primary medium. At this time, the temperature of the primary medium is 95°C, and then slowly add 25ml of sterile defiberized sheep blood to the primary medium to obtain glycerol Lamb's blood chocolate soup, after the glycerin lamb's blood chocolate soup is cooled to room temperature, dispense it into 10ml sterile test tubes, and each test tube contains 5ml glycerin lamb's blood chocolate soup to obtain a chocolate medium;
[0022] In the second step, the Haemophilus strain is preserved, and the Haemophilus standard s...
Embodiment 3
[0026] This Haemophilus strain preservation method includes the following steps:
[0027] The first step is to make the culture medium. Mix 100ml of glycerol and 100ml of distilled water to make the primary culture medium. Then put the primary culture medium into a sterilizer for sterilization. The sterilization temperature is 121°C and the sterilization time is 15 minutes. After the pressure of the pressure gauge of the sterilizer drops to zero, immediately remove the primary medium. At this time, the temperature of the primary medium is 95°C, and then slowly add 25ml of sterile defiberized sheep blood to the primary medium to obtain glycerol Lamb's blood chocolate soup. After the glycerin lamb's blood chocolate soup is cooled to room temperature, it is divided into 10ml sterile test tubes, and each test tube is filled with 5ml glycerin lamb's blood chocolate soup to obtain a chocolate medium;
[0028] In the second step, the Haemophilus strains are stored, and the Haemophilus sta...
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