Bacillus pumilus strain and application thereof in apple alternaria leaf spot prevention and control

A technology of Bacillus pumilus and leaf spot disease, applied in the application, bacteria, fungicides and other directions, can solve the problems of long period of disease-resistant varieties, single resistance, pesticide residues, etc., and achieve good application prospects, fast growth, resistance to Reverse effect

Active Publication Date: 2015-11-04
CHINA KINGDOM AGRITECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues

Method used

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  • Bacillus pumilus strain and application thereof in apple alternaria leaf spot prevention and control
  • Bacillus pumilus strain and application thereof in apple alternaria leaf spot prevention and control
  • Bacillus pumilus strain and application thereof in apple alternaria leaf spot prevention and control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and purification of Bacillus pumilus strain QZ-002H

[0028] Bacillus pumilus bacterial strain QZ-002H of the present invention adopts the dilution plate method and plate streaking method to separate and obtain from the sea mud of Jiaozhou Bay, Qingdao, and the separation method is:

[0029] ⑴Isolation of Bacillus: sea mud collection, select sea mud of different textures in different areas of Jiaozhou Bay, Qingdao, and adopt random sampling method to collect appropriate amount of sea mud with a depth of 10-20cm around and in the center of the plot, and mix them in equal amounts. uniform. Indicate the location, time and person of the collection. Weigh 1g of sea mud sample into 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°...

Embodiment 2

[0040] 1. Fermentation process of Bacillus strain QZ-002H

[0041] LB liquid medium: formula: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0042] A large amount of solid fermentation medium formula (mass percentage): solid material: corn flour 60%, bran 40%; inorganic salt solution: potassium dihydrogen phosphate 3.5%, magnesium sulfate 0.04%, ammonium sulfate 4%, the remainder is water ; The solid-to-liquid ratio is 1:1.8 (mass ratio).

[0043] Mass solid fermentation process of Bacillus pumilus strain QZ-002H:

[0044] ①Cultivation of strains in seed solution: Pick a small amount of bacteria from the slant of the test tube of Bacillus pumilus QZ-002H, transfer them to LB liquid medium, shake and culture at 30°C for 3-5 days, and this is the seed solution.

[0045] ②Cultivation of solid-producing strains: Inoculate the seed liquid into solid medium (500mL Erlenmeyer flask) at a ratio of 10%, and culture at a constant...

Embodiment 3

[0048] Embodiment 3 field test

[0049] In recent years, apple spotted leaf defoliation has become another major disease, some causing severe leaf defoliation in July and August, weakening tree vigor, and affecting fruit yield and quality. The present invention measures the indoor toxicity of the bacillus pumilus strain QZ-002H wettable powder for preventing and controlling the pathogen of apple spot leaf fall disease.

[0050] 1 Test object and source

[0051] The test object is apple spot defoliation caused by a virulent strain of apple ring spot, which overwinters as mycelium on the damaged leaves and branches. In the spring of the following year, pycnidia are produced and conidia are released. The conidia spread with the airflow and infect the young leaves of spring shoots. In July, 2013, the bacteria isolated from the "Daguoguang" apple in Xiuchuan Aijia Village, Licheng District, Jinan City, and the strain code named JL-02. According to the investigation, mancozeb is...

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Abstract

The invention discloses a bacillus pumilus strain and an application thereof in apple alternaria leaf spot prevention and control. The strain is the bacillus pumilus strain QZ-002H, and the preservation number is CGMCC No.11050. The invention further discloses a biocontrol preparation containing the bacillus pumilus strain QZ-002H and a preparation method thereof. Experiments prove that the bacillus pumilus strain has the advantages of being rapid in growth rate, large in sporulation quantity, high in stress resistance, capable of being rapidly colonized in plant rhizosphere in large quantities and the like, and therefore a good application prospect is achieved. The biocontrol preparation prepared from the bacillus pumilus can effectively prevent and control apple alternaria leaf spot.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a strain of Bacillus pumilus capable of efficiently preventing and treating plant diseases and its use, and a biological control agent prepared by using the Bacillus pumilus. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop death, resulting in significant economic losses. For the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. The disease-resistant variety has a long cycle and a single resistance. Chemical control uses a long time and a large amount of pesticides, resulting in pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 栾守业牛赡光牛兆颖刘幸红崔凤云
Owner CHINA KINGDOM AGRITECH QINGDAO
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