Molecular markers and qtl sites of pod enzymes in strong winter Chinese cabbage type winter rapeseed

A cabbage-type winter rapeseed, molecular marker technology, applied in the field of molecular biology and genetic breeding, can solve the problems that the research on molecular markers and QTL positioning has not been reported yet, and achieve the goals of accelerating the breeding process, improving screening efficiency, and saving production costs Effect
CN105018596BActive Publication Date: 2018-08-31GANSU AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GANSU AGRI UNIV
Publication Date
2018-08-31

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Abstract

The invention discloses molecular markers and a QTL site of POD enzyme of strong winter Brassia campestris L. Screening comprises the following steps: (1) constructing an F2-generation segregation population through hybridization of parents obtained through multi-generation bagged self-crossing, i.e., strong winter superstrong-cold-resistance winter rape Longyou No. 7 and strong-cold-resistance winter rape Longyou No. 9; (2) extracting leaf DNAs of the two parents and the F2-generation segregation population by using a CTAB method; (3) carrying out polymorphism primer screening on the DNAs of the two parents by using SSR and InDel primer pairs; (4) constructing a genetic linkage map according to obtained genotype data of the F2-generation segregation population and carrying out QTL analysis; and (5) naming the obtained one QTL site of the POD enzyme of strong winter Brassia campestris L. as Qpod. gsau-10A and acquiring the two molecular marks linked with the QTL site of the POD enzyme of strong winter Brassia campestris L., i.e., O112E03 and BrID90115.
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Description

Technical field

[0001] The invention belongs to the technical field of molecular biology and genetic breeding, and specifically relates to a strong winter cabbage type winter rape POD enzyme activity gene QTL locus, and also relates to a strong winter cabbage type winter rape POD enzyme activity gene QTL locus linkage Molecular markers. Background technique

[0002] Cabbage-type rape has a long history of cultivation in my country and has abundant germplasm resources. It is one of the important oil crops in my country. However, the cabbage type rape is mainly spring, and the strong winter cabbage type winter rapeseed is lacking in germplasm, especially the strong winter rape variety that is suitable for planting in northern arid and cold regions. The climate in northern my country is severely cold, and winter rape has excellent cold resistance to survive the winter safely. Therefore, the selection of cold-resistant varieties is the key to the development of winter rape in northe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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