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A kind of microcapsule and preparation method thereof

A technology of microcapsules and capsule walls, which is applied in the field of protein microcapsules and its preparation, can solve the problems of cumbersome preparation process, microcapsule perforation, breaking, etc., and achieve the effects of increasing application value, good stress resistance, and enhancing insecticidal effect

Inactive Publication Date: 2017-12-12
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this template etching method also has certain limitations.
For example, 1) the etching and purification of the template make the whole preparation process cumbersome and difficult; 2) the target molecule needs to enter the interior of the microcapsule through steps such as infiltration; 3) the template usually needs to be prepared into a specific shape by a special method for subsequent 4) The high mechanical pressure in the capsule generated during the removal of the template easily leads to the perforation and fragmentation of the microcapsules

Method used

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  • A kind of microcapsule and preparation method thereof
  • A kind of microcapsule and preparation method thereof
  • A kind of microcapsule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A) Preparation of core material

[0036] Cry8Ca2 spherical crystal protein and spore mixture extraction process or refer to Ke Luo, DavidBanks, Michael J. Adang.Toxicity, binding, and permeability analyses of fourBacillus thuringiensis Cry1 delta-endotoxins using brush border membranevesicles of Spodoptera exigua and Spodoptera frugiperda.Appl Environ Microb, 1999, 65: 457–464. It should be noted that this method extracts a mixture of crystal protein and spores. Because the spores can also play an insecticidal effect after germination, and they have strong resistance to stress, and at the same time, it does not affect the protein crystals as the core material to be wrapped by the wall material, so there is no need to remove the spores before preparing the microcapsules with crystal protein. However, in this embodiment, spores are removed and pure protein crystals are used as the core material to be wrapped.

[0037] The separation process of crystal protein and spores or r...

Embodiment 2

[0049] Preparation of core material: the process of extracting the mixture of Cry1Ac three-dimensional rhombic crystal protein and spores or refer to KeLuo, David Banks, Michael J. Adang. Toxicity, binding, and permeability analyses of four Bacillus thuringiensis Cry1 delta-endotoxins using brush border membranevesicles of Spodoptera exigua and Spodoptera frugiperda. Appl Environ Microb, 1999, 65:457-464.

[0050] 1.0mg / ml Cry1Ac three-dimensional rhombohedral crystal protein and spore mixture: 50mg Cry1Ac crystal protein and spore mixture was dissolved in 50ml of distilled water. The concentration is the concentration of the protein, and the method for determining the concentration is the protein concentration measured after protein electrophoresis using SDS-PAGE.

[0051] Take 10 ml of the 1.0 mg / ml Cry1Ac crystal protein and spore mixture solution prepared above, centrifuge at 6000 rpm for 20 minutes (min), and discard the supernatant. The precipitate was washed with water and ...

Embodiment 3

[0056] Use 95% DD chitosan and sodium alginate as wall materials, and the others are the same as in Example 2.

[0057] In the end, Cry1Ac crystal protein is used as the core material, and the inner-out (chitosan-sodium carboxymethyl cellulose) 5 The sequence of assembled protein microcapsules. The capsule wall of the protein microcapsule is 10 layers. And the prepared protein microcapsules are doped with spores or spore microcapsules wrapped by wall materials.

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Abstract

The invention relates to the technical field of microcapsules, in particular to a protein microcapsule and a preparation method thereof. The microcapsules use protein as the core material and polyelectrolyte macromolecules with positive and negative charges as the wall material, and the polyelectrolyte macromolecules with positive and negative charges are alternately adsorbed on the outer layer of the core material layer by layer Form the cyst wall. And the invention also provides a method for preparing the microcapsule.

Description

Technical field [0001] The invention relates to the technical field of microcapsules, in particular to a protein microcapsule and a preparation method thereof. Background technique [0002] Microcapsules are considered to be one of the best controlled released carrier tools, especially in biomedicine fields such as drug delivery, biosensors and bioreactors. [0003] There are many methods for preparing microcapsules. The microcapsules prepared by layer by layer self-assembly (LbL) technology can accurately control the size, shape, composition, thickness, and structural morphology of the wall material, and thus have attracted much attention. Due to its novel capsule wall structure, excellent versatility and stimulus responsiveness, nano- and micro-scale capsules prepared by LbL technology also have potential applications in various fields such as bioscience, catalysis, environment, and food. [0004] However, the current LbL microcapsules are generally hollow polyelectrolyte microca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02A01N25/28A01N63/00A01P7/04
Inventor 郭淑元贺晓琳
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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