A method for removing lead-containing sewage pollutants

A technology for pollutants and sewage, applied in botany equipment and methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as ecological environment pollution, human physical and mental health hazards, abnormal operation, etc., and achieve good application foreground effect

Active Publication Date: 2017-10-27
利诚检测认证集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of industrial sewage is relatively complex, especially a large number of artificially synthesized compounds entering the environment. Such substances are mainly ammonia nitrogen, sulfide and phosphorus-containing compounds. Due to the complexity of the structure of these substances, they cannot be decomposed and utilized by microorganisms in a short time. The traditional wastewater treatment method using activated sludge to cultivate and domesticate microorganisms can no longer effectively remove these pollutants. These substances have accumulated in the environment for a long time, causing great pollution to the ecological environment on which we live, and affecting human physical and mental health. bring great harm
A considerable number of industrial polluting enterprises in our country would rather be punished than invest in wastewater treatment. Even if there is a sewage treatment plant, the operation is extremely abnormal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for removing pollutants in sewage, comprising the steps of:

[0027] Preparation of composite microbial agent: mix the mixed bacterial liquid and the carrier according to the weight ratio of 1:1, stir evenly, then leave it to stand for 6 hours, and finally put it in 4°C for low-temperature drying, and control the water content at 6% after drying, to obtain final product The carrier is obtained by mixing bamboo charcoal, chitosan and diatomaceous earth according to the mass ratio of 2:2:1; the above-mentioned mixed bacteria liquid is mixed by the raw material bacteria of the following parts by weight: 10 parts of Rhodococcus, 9 parts of Thiobacillus denitrificans , 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of Bacillus pumilus, 2 parts of Phanerochaete chrysosporium; the concentration of the above-mentioned raw material bacteria is controlled at 1X10 8 pcs / ml.

[0028] Sewage pretreatment: First, the sewage NH3-N is 300mg / L, sulfide is 80...

Embodiment 2

[0031] Use DNAMAN software to design primers, add BamHI and SalI restriction sites respectively, synthesize the nucleotide sequence OXR gene according to the amino acid sequence NP_744952.1 whole gene, obtain the target fragment by amplification, and obtain the target gene by PCR amplification OXR (corresponding mutation sites 11D / S, 59K / Q, 77A / D, 99R / G, 133N / Y, 182L / D, 208A / I, 241M / K, 259V / P, 302A / S, 366V / P, 383S / G, 421P / T, 433F / I were introduced into the gene sequence, thereby obtaining different mutant genes), the PCR product was double-digested with BamHI and SalI, and the PCR product was double digested with BamHI and SalI The digested expression vector pBPSE was connected, and the successfully verified recombinant plasmid was transformed into the Pseudomonas stutzeri CCTCC NO: M209107 to obtain a degraded genetically engineered bacterium.

Embodiment 3

[0032] Example 3 Verification of the sewage treatment effect of Pseudomonas stutzeri genetically engineered bacteria and other bacterial agents

[0033] According to the method of Example 1, the corresponding sewage treatment experiment was carried out, and the sewage and the sewage of Example 1 belonged to the same batch and had the same concentration of pollutants. Wherein each component composition of bacterial agent is identical with embodiment 1.

[0034] The genetically engineered bacteria with different mutation sites prepared in Example 2 were respectively used to carry out the reaction of removing lead. It was found through experiments that 11D / S, 59K / Q, 77A / D, 99R / G, 133N / Y, 182L / D, 208A / I, 241M / K, 259V / P, 302A / S, 366V / P, 383S / G, 421P / T, 433F / I mutants all have significantly enhanced effects compared with the original strain. The total treatment time is not more than 4 days, and the result is as follows: the sewage is the same batch of sewage, so as to ensure the same...

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Abstract

The invention relates to a method for removing lead-containing sewage pollutants, comprising the following steps: first, the sewage is passed through a solid-liquid separator to separate the solid from the liquid to remove bulk solid particulate matter, then the liquid enters a sedimentation tank for 12 hours of precipitation, and then Pass the liquid through the round-hole filter to remove solid flocs, and the liquid filtered through the round-hole filter enters the biological reaction tank, adjust the pH to 7, and add 10 grams of compound bacterial agent per cubic meter of liquid each time, once a day , Continuous dosing for a week, and finally let it stand for 3 days to drain the liquid. The method of the invention can effectively remove pollutants containing ammonia, nitrogen, sulfur, phosphorus and lead, has low input cost, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for removing pollutants in lead-containing sewage. Background technique [0002] With the development of industrial production and the improvement of people's living standards, the volume of industrial sewage and urban domestic sewage is increasing at an alarming rate. One of the reasons for threatening the human living environment. [0003] In order to meet the continuous improvement of the public's requirements for environmental quality, the country has formulated more and more stringent emission standards for nitrogen. Research and development of economical and efficient nitrogen removal treatment technology has become the focus and focus of water pollution control engineering research. Although there are many methods that can effectively remove ammonia, such as physical methods include reverse osmosis, distillation, and soil irrigation; ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/00C02F9/14C02F3/34
Inventor 韦秀胆金钊李柱宏胡磊石邓清周仕源欧晖吴镇
Owner 利诚检测认证集团股份有限公司
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