Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of agv2 type circular virus vp2 soluble protein and preparation method thereof

A circular virus and soluble technology, applied in the field of soluble proteins, can solve the problems of cumbersome cloning process and low efficiency, and achieve the effect of simplifying the cloning process

Active Publication Date: 2016-11-30
YANGZHOU UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But sometimes the cloning process is cumbersome and inefficient due to the inability to find a suitable restriction site

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of agv2 type circular virus vp2 soluble protein and preparation method thereof
  • A kind of agv2 type circular virus vp2 soluble protein and preparation method thereof
  • A kind of agv2 type circular virus vp2 soluble protein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Design and amplify the pGEX-6p-1 linearized vector and the primers for the VP2 gene fragment of the AGV2 virus: the upstream primer for amplifying the pGEX-6p-1 linearized vector is located at position 1034-1047 of the pGEX-6p-1 plasmid; and at the 5' end With an additional 15 bases; the downstream primer for amplifying the pGEX-6p-1 linearized vector is located at positions 916-929 of the pGEX-6p-1 plasmid; and with an additional 15 bases at the 5' end. The upstream primer for amplifying the VP2 gene of the AGV2 virus is located at positions 1-27 of the VP2 gene, and the downstream primer for amplifying the VP2 gene of the AGV2 virus is located at positions 376-399 of the VP2 gene. The additional 15 bases at the 5' end of the upstream primer of the pGEX-6p-1 linearized vector are reverse complementary to the first 15 bases at the 5' end of the VP2 downstream primer; the additional 15 bases at the 5' end of the downstream primer of the pGEX-6p-1 linearized vector The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an AGV2 (avian gyrovirus 2) type soluble VP2 (viral protein 2) and a preparation method thereof. The preparation method comprises the following steps: using a pGEX-6p-1 linearization carrier and a primer of an AGV2 VP2 gene segment; directly and quickly recombining and cloning VP2 in vitro by using recombinase ExnaseTM II without carrying out enzyme digestion ligation reaction; converting escherichia coli; carrying out inducible expression through IPTG, so as to achieve the fusion soluble expression of VP2 of AGV2 and GST and obtain purified soluble VP2. The AGV2 VP2 soluble expression and purified protein obtained through the preparation method can directly provide the soluble VP2 as an AGV2 diagnostic antigen and as an immunogen to obtain an anti-VP2 polyclonal antibody, provides an effective immunology reagent for carrying out AGV2 serology epidemiology researches and VP2 functional study, fills up the blank at home and abroad, and has an important meaning for further exploring VP2 biological function.

Description

technical field [0001] The invention relates to a soluble protein, in particular to an AGV2 type orbivirus VP2 soluble protein and a preparation method thereof. Background technique [0002] Chicken infectious anemia virus (CAV) has been considered the only member of the circovirus genus. Until 2011, Rijsewijk et al. detected a new type of Circovirus sequence, the second member of the Circovirus genus, named AGV2 from serum samples of infected chickens. In the same year, Sauvage et al. detected the first sequence of a new type of human circular virus HGyV in skin cotton samples of healthy people. Sequence analysis surprisingly found that the genome sequence homology between HGyV and AGV2 was as high as 96%. Recently, Maggi and Biagini et al. also detected DNA sequences of HGyV / AGV2 in serum samples of healthy people, organ transplant patients and HIV-positive patients. In China, Ye Jianqiang and others detected and reported the existence of AGV2 in chicken flocks and huma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/01C12N15/70C12R1/19
CPCC07K14/01C07K2319/23C12N2750/10011
Inventor 叶建强田晓彦施洋洋邵红霞秦爱建谢泉夏驰超周晓祥范中雷
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com