Detection method for multiple amino acids in food

A detection method and amino acid technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems that the detection data cannot fully represent the protein content of the product and cannot be distinguished, and achieve the effect of strong versatility and avoiding interference.

Active Publication Date: 2015-11-25
北京市产品质量监督检验院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many disadvantages in this method. The test data cannot fully represent the protein content of the product, and it is impossible to distinguish whether it is the protein content in the product or artificially added high-nitrogen substances, such as melamine.

Method used

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  • Detection method for multiple amino acids in food
  • Detection method for multiple amino acids in food
  • Detection method for multiple amino acids in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0069] (1) Standard solution preparation

[0070] Select a mixed standard solution of 31 kinds of amino acids, which are: taurine, urea, aspartic acid, hydroxyproline, threonine, serine, asparagine, glutamic acid, sarcosine, Glycine, Alanine, Citrulline, Alpha-Aminobutyric Acid, Valine, Cystine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Beta-Alanine , β-aminobutyric acid, γ-aminobutyric acid, tryptophan, ethanolamine, hydroxylysine, ammonia, lysine, histidine, carnosine, arginine; concentration of amino acid mixed standard solution: 0.25μmol / mL (Pickering, calibration standard); successively dilute with eluent into five gradients: 0.005 μmol / mL, 0.0125 μmol / mL, 0.025 μmol / mL, 0.05 μmol / mL, 0.25 μmol / mL.

[0071] (2) Measurement conditions

[0072] (1) Chromatographic conditions:

[0073] A. The chromatographic column adopts:

[0074] Pre-column: cation-exchange lithium guard column, inner diameter 2.0×20mm, 5 μm (purchased from Pickering Laboratories Catal...

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Abstract

The invention provides a detection method for a plurality of amino acids in food and application of ninhydrin post-column derivatization-high performance liquid chromatography in detection of a plurality of amino acids in wines. The detection method is characterized in that a high performance liquid chromatograph and an amino acid post-column derivatization apparatus are used to detect a plurality of amino acids in food under specific chromatographic conditions and post-column derivatization conditions. The method provided by the invention can detect more than 30 amino acids in food at the same time, has good versatility and is applicable to detection of amino acids in different foodstuffs. Moreover, derivative reaction takes place after separation of amino acids from other substances, so interference by other substances is avoided, and the method is applicable to analysis of a plurality of amino acids in an unknown complex sample.

Description

technical field [0001] The invention relates to a method for detecting multiple amino acids in food and the application of ninhydrin post-column derivatization-high performance liquid chromatography in detecting multiple amino acids in wine. Background technique [0002] In recent years, China's food safety issues have emerged frequently. In the "artificial honey" incident in 2007, some criminals added thickeners, sweeteners, preservatives, flavors and pigments and other chemicals to fake honey. The fake honey had almost no nutritional value; the illegal addition of industrial raw materials in 2008: "Sudan red duck eggs", "melamine milk". The "melamine" incident was a food safety incident that shocked the world. In the immediate storm of industry purges, the public had to accept the fact that quality problems caused by milk sources generally existed in the entire industry, and the problems of large enterprises and well-known brands were particularly concentrated. , China's...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 吴颖
Owner 北京市产品质量监督检验院
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