Protein concentration detection kit and operation method thereof
A technology of concentration detection and operation method, applied in the field of analysis, can solve the problems of protein sample waste, time-consuming and laborious, and large sample volume, and achieve the effect of avoiding waste, detecting significant significance, and reducing the amount of sample added.
Inactive Publication Date: 2015-11-25
TIANJIN QIANTAI BIOTECH CO LTD
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Problems solved by technology
[0002] At present, in the process of detecting protein concentration, especially when using G-250 chromogenic hair to detect protein concentration, spectrophotometer is generally used for detection, and each sample must be detected separately, and the concentration of each sample must be detected during detection. The amount of sample required is also large, this method of operation is time-consuming and laborious, and causes a certain amount of waste to some valuable protein samples
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example 1
[0026] Example 1: Determination of extracellular protein content produced by beer yeast at different fermentation times
[0027] Take brewer's yeast fermentation liquid supernatant at different fermentation times, take 20 microliters respectively and add to 96-well microtiter plate. The sampling time is: 12 hours, 24 hours, 36 hours, 48 hours, 60 hours, 72 hours, 84 hours, 96 hours.
[0028] Take out the bovine serum albumin standard solution prepared in advance from the refrigerator, melt it, and add 20 microliters of standard solutions of different concentrations into the 96-well microtiter plate.
[0029] Take 20 microliters of distilled water and add it to a 96-well microtiter plate as a blank control.
[0030] Add 150 microliters of AB mixed solution to the wells of the 96-well microplate plate where the samples were added, and let stand for 5 minutes to detect with a microplate reader with a wavelength of OD595nm. The test results of the standard curve are shown in T...
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The invention relates to the field of protein concentration detection and provides a batch protein concentration detection kit and an operation method thereof. The kit comprises a 96-well ELISA plate, a standard substance bovine serum albumin, a solution A, a solution B, a cleaning liquid and several 0.5 ml EP tubes. Through combination of solutions in the kit, a light absorption value is produced at a certain wavelength, according to the above principle, an unknown protein concentration is detected by a standard substance as a reference substance, and large scale and fast detection can be realized on the 96-well ELISA plate. The batch protein concentration detection kit prevents detection time difference-caused errors.
Description
technical field [0001] The invention belongs to the technical field of analysis. In particular, it relates to a protein concentration detection kit and an operation method. Background technique [0002] At present, in the process of detecting protein concentration, especially when using G-250 chromogenic hair to detect protein concentration, spectrophotometer is generally used for detection, and each sample must be detected separately, and the concentration of each sample must be detected during detection. The amount of sample required is also large, and this method of operation is time-consuming and laborious, and causes a certain amount of waste to some precious protein samples. Contents of the invention [0003] In view of this, the purpose of the present invention is to overcome the deficiencies in the prior art, provide a kind of batch protein concentration detection kit and operation method, can realize the detection of large-scale protein sample in a short time by ...
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IPC IPC(8): G01N33/68G01N33/53
CPCG01N33/6803G01N33/53
Inventor 张涛田方王巍
Owner TIANJIN QIANTAI BIOTECH CO LTD