Organic matter enrichment and concentration method based on chemical and biological analysis of low-pollution water body
A technology for biological analysis and organic matter, applied in the direction of analytical materials, scientific instruments, material separation, etc., can solve the problems of only considering recovery, can not guarantee recovery rate, unfavorable biological toxicity detection, qualitative and quantitative analysis of organic physical and chemical indicators, etc., to reduce water The effect of increasing the sample volume and improving the sensitivity
Inactive Publication Date: 2020-05-19
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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Problems solved by technology
However, effective and accurate detection requires scientific and efficient water sample pretreatment steps
At present, the pretreatment of water samples often only considers the recovery of trace organic pollutants, but does not consider the recovery of macromolecular organic substances; or only considers the recovery of macromolecular organic substances, but cannot guarantee an effective recovery rate
Therefore, it is not conducive to the subsequent qualitative and quantitative analysis of biological toxicity detection and organic physical and chemical indicators.
Method used
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Embodiment 1
[0040] Such as figure 1 As shown: According to the standard method, a 20L secondary sedimentation tank effluent water sample was collected from a typical sewage treatment plant, of which 10L water sample was carefully processed according to the above-mentioned inventive method; the other 10L water sample was carefully sample processed according to the commonly used methanol method: A certain amount of sewage water sample is filtered through a 0.7μm glass fiber membrane; the HLB column is activated and cleaned with 20mL methanol and 10mL ultrapure water; the water sample is continuously passed through the column to keep the flow rate of the water sample at 5mL / min Below, after the sewage water sample is loaded, use 10mL ultrapure water to clean the column; use the solid phase extraction device to continue vacuum pumping for 20 minutes to fully dry the column, and then put the column into a centrifuge for further dehydration and drying ; Use 20mL methanol solution to elute the s...
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The invention relates to an organic matter enrichment and concentration method based on the chemical and biological analysis of a low-pollution water body. The method comprises the following steps: acidifying a sewage sample, and adding an organic solvent methanol into the water sample to optimize the retention conditions of organic matters in the sewage in a subsequent solid-phase extraction small column; by adjusting the type and volume ratio of the organic solvent, optimizing the adsorption condition and subsequent elution condition of the solid-phase extraction small column on organic matters in the sewage; by adjusting the solvent of the organic matter, optimizing the dissolving condition of an obtained concentrated organic matter. According to the method, the sewage total organic matter recovery rate can reach 85% or above, and the enriched and concentrated organic matter can be used for subsequent quantitative analysis of a trace organic matter and qualitative analysis of a biological effect at the same time. The method comprehensively considers the recovery of micromolecular organic matters and macromolecular organic matters in the sewage, has the advantages of high accuracy and favorable recovery rate, and provides a scientific and effective water sample pretreatment method for monitoring trace organic pollutants and biotoxicity effects in the sewage.
Description
technical field [0001] The invention relates to the technical field of detection of environmental water bodies, in particular to a method for enriching and concentrating organic matter based on chemical and biological analysis of low-pollution water bodies. Background technique [0002] The dissolved organic matter in the secondary effluent of the sewage plant accounts for more than 90% of the total organic matter. There are many types of such substances and their properties are miscellaneous. They include trace organic pollutants of small molecules, as well as substances such as macromolecular polysaccharides, proteins, and humic acids. . At present, trace organic pollutants detected in the secondary effluent of sewage treatment plants include endocrine disruptors (EDCs), pharmaceuticals and personal care products (PPCPs), organophosphorus flame retardants (OPFRs) and pesticides, etc. Substances in the receiving water body of sewage may produce bioaccumulation effects on t...
Claims
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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N1/40
CPCG01N30/02G01N30/06G01N30/14G01N1/40G01N1/4055G01N1/4077G01N2030/062G01N2030/146
Inventor 马晓妍王永坤唐磊王晓昌姚宇贾燕茹
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY