A method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube plantlets

A technology of test-tube seedlings and paulownia, applied in the field of plant cultivation, can solve the problems of low cultivation efficiency and achieve the effects of thorough disinfection, promotion of growth and branching, and small variation rate

Active Publication Date: 2018-07-17
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to provide a method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube seedlings, so as to solve the problem of low cultivation efficiency in the prior art

Method used

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  • A method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube plantlets
  • A method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube plantlets
  • A method for large-scale, high-efficiency and rapid propagation of Paulownia test-tube plantlets

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Effect test

Embodiment 1

[0025] A method for large-scale, high-efficiency and rapid propagation of paulownia test-tube seedlings includes the following steps:

[0026] (1) Knead the seeds of Paulownia to remove the membranous wings of the seeds, wrap the seeds in a gauze bag and tie the bag mouth, rinse in running water for 10 minutes, wash with household detergent for 10 minutes, rinse with running water for 20 minutes, and squeeze dry The water is brought into the ultra-clean workbench, and the ultra-clean workbench is soaked in 75% volume fraction alcohol for 45-60s, 0.1% mercury liters soaked for 6-8min, and rinsed with sterile water for 5-7 times. Then cut the gauze bag, use tweezers to pick out the seeds, and set aside.

[0027] (2) The paulownia tree seeds obtained in step (1) are sown in a solid medium, and cultivated for 20-30 days under the conditions of 22~25℃, illumination 500~1000 Lux, and illumination time 11~13h / d, and the seeds germinate Form 4-5cm young and weak seedlings, with a germinat...

Embodiment 2

[0032] Basically the same as Example 1, in step (2), step (3), and step (4), the three stages of seed germination, sub-generation culture and strong seedling rooting culture were screened with the medium combination listed in Table 1 respectively. The optimal medium combination formula is preferred. Table 1 explains as follows:

[0033] ①The optimization of medium formula is mainly to design different basic medium, hormone concentration, etc. to optimize the medium design. In this example, all are designed as solid medium. The agar concentration in each formula is 6g / L and sucrose is 30g. / L, the culture environment is a culture room with a temperature of 25°C, an illumination of 1000-2000 lux, and an illumination time of 12h / d. ②Different medium formulas are designed for different cultivation stages. The seed sowing stage mainly observes the speed of seed germination, growth, color (green is better) and other indicators to judge whether the formula is reasonable; the sub-genera...

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Abstract

The invention discloses a method for efficiently and rapidly reproducing paulownia test-tube plantlets in a large scale. The method comprises the following steps: kneading paulownia seeds to remove membranous wings, charging the paulownia seeds into a gauze bag, bleaching and sterilizing, then inoculating into a solid culture medium, culturing for 20 to 30 days, and facilitating the sprouting of the seeds to obtain seedlings; cutting the young seedlings into sections with a node, transferring the seedlings into a reproduction subculture medium, culturing for 30 to 40 days, subculturing for one time, wherein the reproduction coefficient reaches 4 to 5, when a needed seed plantlet quantity is achieved, transferring to a seedling improving and rooting culture medium, culturing for 40 to 50 days, the seedling growing strong and having 3 to 4 roots, and transplanting the seedling. Compared with the prior art, the method can be directly used for the factory large-scale production of seed plantlets and has advantages of less pollution, high reproduction speed, low aberration rate, tidy and strong seed plantlets, low production cost, high transplanting survival rate and the like.

Description

Technical field [0001] The invention belongs to the technical field of plant cultivation, and specifically relates to a method for large-scale, high-efficiency and rapid propagation of paulownia test-tube seedlings. Background technique [0002] The genus Paulownia (Paulownia Sieb.et Zucc.) is mostly deciduous trees, and the tropics are evergreen. There are 7 species of this genus, all of which are produced in China. They are distributed all over the country except for the northern northeast, Inner Mongolia, northern Xinjiang, and Tibet. Paulownia is a specialty tree species in China with strong fast-growing properties. It is an important tree species for plain greening, construction of farmland shelterbelts, tree planting on the sides, and forest-grain intercropping. Paulownia wood has straight texture, beautiful pattern, pleasing color, light and soft material, low density, stable size, no warping or cracking, and it is the best material for the production of single boards suc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 史俊徐超蒋新宇汪善锋杨鹤同席刚俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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