Method utilizing nano-channel sensor modified by nucleic acid probe having super sandwich structure to high-sensitively and high-specifically detect Zn2+

A technology of sandwich nucleic acid and nano-channel, which is applied in the direction of electrochemical variables of materials, can solve the problems of high preparation cost and limited scope of use, and achieve the effect of popularization and application, universality and good stability.

Active Publication Date: 2015-12-09
江苏巨珩新材料科技有限公司
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Problems solved by technology

Although the fluorescent probe method has the advantages of simple operation and rapidity, the synthesis and modification of the fluorescent dye used in the detection process ...

Method used

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  • Method utilizing nano-channel sensor modified by nucleic acid probe having super sandwich structure to high-sensitively and high-specifically detect Zn2+
  • Method utilizing nano-channel sensor modified by nucleic acid probe having super sandwich structure to high-sensitively and high-specifically detect Zn2+
  • Method utilizing nano-channel sensor modified by nucleic acid probe having super sandwich structure to high-sensitively and high-specifically detect Zn2+

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Embodiment

[0022] (1) Preparation of polymer nanopores

[0023] The polymer polyethylene terephthalate (PET) film with heavy ion bombardment tracks is first irradiated for one hour on the front and back sides under a UV lamp to make it more stable in chemical etching . When etching, immerse the irradiated PET film in 2M / L NaOH solution, and maintain the temperature at 50±5°C, take out the PET film at different intervals, rinse the NaOH solution on the surface with deionized water, and then soak In deionized water for 4 hours to remove residual NaOH in the pores and on the surface, an etched PET film containing nanopore arrays was obtained. When the etching time was 6 min, the nanopores were characterized by a scanning electron microscope, and columnar nanopores were obtained. Through statistics on the pore diameter of the pore under the same conditions, the pore diameter of the constructed nanopore is about 80nm.

[0024] (2) has a pair of Zn 2+ Design of super sandwich nucleic acid ...

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Abstract

The invention disclose a method utilizing a nano-channel sensor modified by a nucleic acid probe having a super sandwich structure to high-sensitively and high-specifically detect Zn2+. The method comprises the following steps: (1) preparing a polymer nano channel; (2) designing a nucleic acid probe with a super sandwich structure, wherein the probe has a specific recognition function on Zn2+; (3) carrying out functional modification on the nano channel; (4) carrying out Zn2+ quantitative detection. The invention provides a novel method for detecting specific ions. The detection sensitive of Zn2+ can reach 1 nM, the specificity is high, other ions can be well identified, moreover, the sensor has a stable property, and the repeatability is high.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a method for detecting Zn with high sensitivity and high specificity using a super sandwich nucleic acid probe nanopore sensor. 2+ Methods. Background technique [0002] Zinc is one of the essential trace elements for the human body and an important substance for maintaining the normal growth, development and metabolism of organisms. Scientific research has shown that zinc ions are one of the important metal ions that make up more than 300 kinds of biological enzyme activity catalytic centers; it can be used as a structural factor or transcription factor of metalloproteases; It plays a very important role in the central nervous system. However, when zinc ions are excessively present in the human body, the immunity of the human body will be reduced, which can induce the reduction of iron content in the blood, kidney and liver in the body, and the lack of copper ions, thus cau...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 陈伟陈玉英
Owner 江苏巨珩新材料科技有限公司
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