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Electroporation method and equipment

An electroporation and electrode technology, which is applied in the field of cell electroporation, can solve the problems of cell membrane damage, electroporation cell survival rate and economic efficiency, and cell differentiation.

Active Publication Date: 2018-01-23
陈剑
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the cell membrane may be irreversibly damaged when the electric field strength is too high
Second, while the perforation of the cell membrane produced by the electric field can allow target substances to enter the cell, these perforations may also allow the outflow of substances inside the cell or the uncontrolled inflow of other substances outside the cell, resulting in a negative impact on cell activity.
Third, the heat generated by the current may damage the cells
[0006] While there have been multiple previous efforts to improve electroporation efficiency, the key issue of cellular variability has remained unresolved
The efficiency, cell viability and economy of electroporation need to be improved

Method used

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  • Electroporation method and equipment
  • Electroporation method and equipment

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Embodiment Construction

[0033] Exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. Wherever possible, the same reference numbers will be used in the drawings to refer to the same or like parts.

[0034] Figure 1A An exemplary electroporation device 100 is shown consistent with the disclosed embodiments. The device 100 includes a sample container 10 . The sample container 10 includes an insulating chamber body 14, a first electrode 15a and a second electrode 15b. In the sample container 10 , an interface is formed on the surface of the lower medium 12 and the lower surface of the upper medium 13 . A cell monolayer 11 across the current field can be formed on the interface. A cell monolayer as used in the present invention refers to a single, tightly packed layer of cells. A cell monolayer is therefore also sometimes referred to as a compact cell monolayer or a compact monolayer. Apparatus 100 also includes a pulse generator ...

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Abstract

The invention provides a cell electroporation device. The device includes a sample container having an insulating chamber for containing cells. The sample container has a first electrode and a second electrode to realize electrical connection during electroporation. The insulating chamber is configured to contain at least one cell monolayer. The device also includes a pulse generator to generate preset pulses for cell electroporation.

Description

technical field [0001] The present invention mainly relates to the method and equipment related to cell electrical stimulation, especially the method and equipment of cell electroporation. Background technique [0002] Electroporation is a widely used method of permeabilizing cell membranes in which cells are temporarily perforated by electrical stimulation. The application of electroporation includes the delivery of DNA (deoxyribonucleic acid), RNA (ribonucleic acid), siRNA (small interfering RNA), peptides, proteins, antibodies, drugs or other substances to a variety of cells, such as mammalian cells, plant cells, Yeast, other eukaryotic cells, bacteria, other microorganisms, and human cells. Electrical stimulation can also be used in cell fusion to obtain hybridoma cells or other fused cells. Cell electrofusion can be regarded as a special kind of cell electroporation. [0003] Typically, during electroporation, cells are suspended in a buffer or medium that favors cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12N13/00
CPCA01K67/0278A01K2217/072C12M35/02C12N13/00C12M1/10C12M1/42C12N15/87
Inventor 陈剑
Owner 陈剑