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Pig feed additive

A technology of feed additive and marine red yeast, which is applied in animal feed, animal feed, application, etc., can solve the problems of low pig absorption rate, high excretion rate, and environmental pollution, and achieve good compatibility, low excretion rate, and environmental pollution. small effect

Inactive Publication Date: 2015-12-16
TIANJIN ZHONGTIAN JINGKE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in adding this inorganic copper sulfate to the feed, such as low absorption rate of pigs, poor compatibility with other components in the diet, high excretion rate, and environmental pollution.

Method used

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  • Pig feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance

[0039] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and cultivation. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator....

Embodiment 2

[0056] Example 2: (Comparative Test) Fermentation Conditions for High-yielding Carotenoids of Rhodotorula marina WYN1

[0057] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , the inoculum amount was 10%, the shaker speed was 180r / min, and after culturing at 28°C for 24 hours, the temperature was adjusted to 30°C, the pH was adjusted to 5, and the nitrogen source was added once, that is, peptone was added according to the addition amount of 2.5%, and continued Ferment for 24h. The marine Rhodotorula WYN1 was fermented and cultured by the above method, and its biomass, carotenoid production and intracellular copper content were 27.52 g / L, 25.12 mg / L and 6240 μg / g dry bacteria, respectively.

Embodiment 3

[0058] Embodiment 3: feed additive and its preparation

[0059] The composition of the feed additive is as follows:

[0060] Perilla extract 15%, Eucommia leaf extract 15%, marine red yeast fermentation culture 35%, ganoderma lucidum solid fermentation culture 25%, phytosterol 10%, the marine red yeast preservation number is CCTCCNo: M2014592, The above ratios are all percentages by mass.

[0061] The preparation method of ganoderma lucidum solid fermentation culture is as follows:

[0062] (1) Activation culture of slant strains: transfer the preserved ganoderma lucidum slant strains to the slant medium, and cultivate at 25°C for about 110 hours until the mycelia cover the slope;

[0063] (2) Liquid first-level seed culture: pick a piece of the above-mentioned Ganoderma lucidum slant strain and insert it into a 500-milliliter shaker flask equipped with 100 milliliters of medium to carry out first-level seed culture, culture conditions: rotary shaker 100 rpm, Cultivate at 2...

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Abstract

The invention provides a pig feed additive. The pig feed additive comprises 10-20% of perilla frutescens extract, 10-20% of eucommia bark leaf extract, 30-40% of rhodotorula sp. fermentation culture, 20-35% of ganoderma lucidum solid fermentation culture and 10-15% of phytosterol by mass, wherein the collection number of rhodotorula sp. is CCTCC No:M 2014592. The pig feed additive has the beneficial effects that rhodotorula sp. can produce high-yield carotenoids and enrich copper; the carotenoid yield and copper content in rhodotorula sp. are effectively increased by a static culture method; and applied to feeds as a feed additive, rhodotorula sp. can provide carotenoids, B vitamins and copper ions, well replaces inorganic copper ions used in conventional feeds thanks to efficient copper enrichment capacity, has good stability, has good compatibility with other components in the diets, has a high absorption rate and a low excretion rate, is little in environmental pollution and has the effects of improving the skins and hair colors of animals and strengthening the nutritional values of the feeds.

Description

technical field [0001] The invention belongs to the field of feed additives, in particular to a pig feed additive product and a preparation method thereof. Background technique [0002] In the process of modern livestock and poultry farming, in order to prevent and treat animal diseases, excessive antibiotic products are often used to ensure the health of animals, but the excessive use of antibiotics often leads to the decline in the quality of animal meat products and their products and the enhancement of antibiotic resistance, resulting in transmission through the food chain. Human health is also severely affected by the problem of antibiotics. Some natural substances derived from nature not only have good nutritional properties, but also have anti-virus, anti-inflammatory, anti-oxidation, and regulation of immune functions, etc., and have broad application prospects. [0003] Ganoderma Lucidum (Leyss.exFr.) Karst.], also known as Ganoderma lucidum, belongs to Basidiomyco...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/14A23K1/00A23K1/16
Inventor 李建树
Owner TIANJIN ZHONGTIAN JINGKE SCI & TECH
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