Pig feed additive

A technology of feed additive and marine red yeast, which is applied in animal feed, animal feed, application, etc., can solve the problems of low pig absorption rate, high excretion rate, and environmental pollution, and achieve good compatibility, low excretion rate, and environmental pollution. small effect

Inactive Publication Date: 2015-12-16
TIANJIN ZHONGTIAN JINGKE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in adding this inorganic copper sulfate to the feed, such as low absorption rate

Method used

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  • Pig feed additive

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1: Acquisition of marine Rhodotorula WYN1 and domestication of copper tolerance

[0039] (1) Fresh prawns, starfish, seaweed and other biological samples will be collected from the Yellow Sea and placed in a sterile YEPD medium, and red or pink round colonies will be picked for further purification and culture. According to the accumulation of carotenoid of each type of bacteria, the bacteria with high carotenoid production were selected. Among them, the marine red yeast WYN1, which produces higher carotenoids, has oval cells without pseudohyphae; red colonies are formed on YEPD medium with seawater, with smooth surfaces and neat edges; no seeds are produced on sporulation medium Cystospores, multilateral budding, no spore-bore; no sugar fermentation, no starch-like compounds, different inositol. Based on the above characteristics, it is preliminarily determined to belong to the genus Rhodotorula. Keep the slope in the refrigerator.

[0040] (2) Fermentation cultu...

Example Embodiment

[0056] Example 2: (Comparative test) Research on fermentation conditions of marine red yeast WYN1 with high-yield carotenoids

[0057] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , The inoculation amount is 10%, the rotating speed of the shaker is 180r / min, after 24 hours of incubation at 28°C, the temperature is adjusted to 30°C, the pH is adjusted to 5, and the nitrogen source is added once, that is, peptone is added at the amount of 2.5%, continue Fermentation for 24h. Using the above method to ferment and culture marine red yeast WYN1, its biomass, carotenoid production and intracellular copper content were 27.52g / L, 25.12mg / L and 6240μg / g dry bacteria, respectively.

Example Embodiment

[0058] Example 3: Feed additives and their preparation

[0059] The composition of feed additives is as follows:

[0060] Perilla extract 15%, Eucommia ulmoides leaf extract 15%, marine red yeast fermentation culture 35%, Ganoderma lucidum solid fermentation culture 25%, phytosterol 10%, the marine red yeast preservation number is CCTCCNo: M2014592, The above ratios are all percentages by mass.

[0061] The preparation method of Ganoderma lucidum solid fermentation culture is as follows:

[0062] (1) Slant strain activation culture: transfer the preserved Ganoderma lucidum slant strain to the slant medium, culture at 25°C for about 110 hours, until the hyphae grows over the slant;

[0063] (2) Liquid primary seed culture: Pick a piece of the above-mentioned Ganoderma lucidum slant strain and put it into a 500 ml shake flask containing 100 ml of culture medium for primary seed culture. Cultivation conditions: Rotary shaker 100 rpm, Incubate at 26°C for about 100 hours; incubate at 26°C...

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Abstract

The invention provides a pig feed additive. The pig feed additive comprises 10-20% of perilla frutescens extract, 10-20% of eucommia bark leaf extract, 30-40% of rhodotorula sp. fermentation culture, 20-35% of ganoderma lucidum solid fermentation culture and 10-15% of phytosterol by mass, wherein the collection number of rhodotorula sp. is CCTCC No:M 2014592. The pig feed additive has the beneficial effects that rhodotorula sp. can produce high-yield carotenoids and enrich copper; the carotenoid yield and copper content in rhodotorula sp. are effectively increased by a static culture method; and applied to feeds as a feed additive, rhodotorula sp. can provide carotenoids, B vitamins and copper ions, well replaces inorganic copper ions used in conventional feeds thanks to efficient copper enrichment capacity, has good stability, has good compatibility with other components in the diets, has a high absorption rate and a low excretion rate, is little in environmental pollution and has the effects of improving the skins and hair colors of animals and strengthening the nutritional values of the feeds.

Description

technical field [0001] The invention belongs to the field of feed additives, in particular to a pig feed additive product and a preparation method thereof. Background technique [0002] In the process of modern livestock and poultry farming, in order to prevent and treat animal diseases, excessive antibiotic products are often used to ensure the health of animals, but the excessive use of antibiotics often leads to the decline in the quality of animal meat products and their products and the enhancement of antibiotic resistance, resulting in transmission through the food chain. Human health is also severely affected by the problem of antibiotics. Some natural substances derived from nature not only have good nutritional properties, but also have anti-virus, anti-inflammatory, anti-oxidation, and regulation of immune functions, etc., and have broad application prospects. [0003] Ganoderma Lucidum (Leyss.exFr.) Karst.], also known as Ganoderma lucidum, belongs to Basidiomyco...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/14A23K1/00A23K1/16
Inventor 李建树
Owner TIANJIN ZHONGTIAN JINGKE SCI & TECH
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