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A kind of bacillus amyloliquefaciens and its application for preventing and treating cotton verticillium wilt

A technology of amyloliquefaciens spores and cotton verticillium wilt, which is applied in the field of control of cotton verticillium wilt, and can solve the problems such as no report of Bacillus amyloliquefaciens strain HMB27634 found

Active Publication Date: 2018-08-21
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After searching, no report about Bacillus amyloliquefaciens bacterial strain HMB27634 of the present invention was found

Method used

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  • A kind of bacillus amyloliquefaciens and its application for preventing and treating cotton verticillium wilt
  • A kind of bacillus amyloliquefaciens and its application for preventing and treating cotton verticillium wilt
  • A kind of bacillus amyloliquefaciens and its application for preventing and treating cotton verticillium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1HMB27634 microbial bacterial agent

[0057] Follow the steps below:

[0058] (1) Strain activation: The bacterial strain HMB27634 (Bacillus amyloliquefaciens strain HMB27634) stored at -80°C was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on September 29, 2015, and the preservation number is CGMCC No. 11455) is activated at 30°C on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), and pick a single colony on LB slant medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) was cultivated at 30°C for 12 hours to obtain an activated bacterial strain;

[0059] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, so...

Embodiment 2

[0062] Example 2 Inhibition of Verticillium dahliae spore germination by Bacillus amyloliquefaciens HMB27634

[0063] (1) Test time and place: In June 2013, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0064] (2) Test method:

[0065] (1) Source of Verticillium dahliae for testing: Verticillium dahliae WX-1 strain was collected from a cotton Verticillium dahliae disease strain in Wei County, Xingtai City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Plant Pathology Department of the Conservation College identified it as Verticillium dahaliae, and the pathogenicity test showed strong pathogenicity.

[0066] (2) plate determination test:

[0067] First Verticillium dahliae WX-1 was activated on the PDA plate, and after 7 days of cultivation, 4-5 pieces of mycelium piece...

Embodiment 3

[0071] Example 3 Inhibition test of Bacillus amyloliquefaciens HMB27634 on Verticillium dahliae hyphae growth

[0072] (1) Test time and place: In July 2013, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0073] (2) Test method:

[0074](1) Source of Verticillium dahliae for testing: Verticillium dahliae WX-1 strain was collected from a cotton Verticillium dahliae disease strain in Wei County, Xingtai City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The Plant Pathology Department of the Conservation College identified it as Verticillium dahaliae, and the pathogenicity test showed strong pathogenicity.

[0075] (2) plate determination test:

[0076] First, Verticillium dahliae WX-1 was activated on the PDA plate, and after 7 days of cultivation, in the edge area of ​​the c...

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Abstract

The invention belongs to the field of agricultural biological control, and particularly discloses a Bacillus amyloliquefaciens strain HMB27634 for controlling cotton diseases, of which the collection number is CGMCC No.11455. The invention also discloses a microbial inoculant prepared from the strain and application of the strain and microbial inoculant in controlling verticillium wilt of cotton. The Bacillus amyloliquefaciens has high drug effect on verticillium wilt of cotton, high average control effect (80.0% or above) and high pertinence. The microbial inoculant is safe for human and livestock, and produces less environmental pollution. Besides, when the microbial inoculant is used for controlling verticillium wilt of cotton, the duration time is long, and the tomato can not easily generate drug resistance. The strain is simple in preparation method, low in cost and simple to use.

Description

technical field [0001] The invention belongs to the field of agricultural biological control, and in particular relates to a bacillus amyloliquefaciens used for preventing and treating cotton disease, a microbial agent containing the bacillus amyloliquefaciens, and also relates to their application in preventing and treating cotton verticillium wilt. Background technique [0002] Cotton Verticillium dahliae is a soil-borne disease that is caused by Verticillium dahaliae and is difficult to control. Cotton verticillium wilt often breaks out and causes disasters in our country. The cotton planting area affected by it is more than 40 million mu every year, causing a reduction in cotton production by about 10%-20% all the year round, and the production reduction can be as high as 60%- 70%. [0003] The methods of preventing and treating Verticillium wilt of cotton mainly include: one is chemical control, but the effect of chemical control is relatively poor, and it is easy to c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12Q1/68C12R1/07
Inventor 马平鹿秀云李社增郭庆港张晓云年冠臻王莹
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI