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Fusarium wilt resistance gene tightly linked molecular marker sifwr2145 in sesame

A molecular marker, fusarium wilt resistance technology, applied in the field of molecular genetics and breeding, can solve the problems that the disease resistance level is easily affected by the environment such as temperature and rainfall, and the research progress of disease resistance molecular genetics and molecular assisted breeding is not obvious enough, and the detection method is achieved. Simple and efficient, saving scientific research cost and time, and stable test results

Active Publication Date: 2017-12-01
HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, since the resistance to Fusarium wilt of sesame is a quantitative trait, and the level of disease resistance is easily affected by the environment such as temperature and rainfall, the research progress of molecular genetics and molecular assisted breeding of disease resistance is not obvious enough.

Method used

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  • Fusarium wilt resistance gene tightly linked molecular marker sifwr2145 in sesame
  • Fusarium wilt resistance gene tightly linked molecular marker sifwr2145 in sesame
  • Fusarium wilt resistance gene tightly linked molecular marker sifwr2145 in sesame

Examples

Experimental program
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Embodiment 1

[0045] This example mainly introduces the SNP molecular markers closely linked to the sesame resistance gene SiFWR2145 The screening process is as follows.

[0046] 1. F of sesame resistant and susceptible parents 2 genetic population construction

[0047] In July 2013, a hybrid combination was prepared using the sesame line Yuzhi DS899 (DI 6.1%) with high resistance to Fusarium wilt (DI 6.1%) and germplasm JS012 (DI 100%) with high susceptibility to Fusarium wilt to obtain F 1 .

[0048] In November 2013, the F1 seeds were sown on-demand in a nutrient bowl and planted in the Sanya Base of the Sesame Research Center of the Henan Academy of Agricultural Sciences. After 2 pairs of true leaves, the plants were transplanted in due course to ensure that the number of lines was greater than 200.

[0049] Two, F 2 Population SNP Genetic Map Construction and Gene Mapping

[0050] (1) F 2 Group parent and 122 F 2 Single genome resequencing

[0051] from F 2 120 lines were rand...

Embodiment 2

[0062] Example 2: Molecular markers closely linked to resistance to Fusarium wilt in sesame SiFWR2145 verification of

[0063] This example mainly introduces the molecular markers closely linked with sesame resistance to Fusarium wilt SiFWR2145 The verification, that is, the detection method of the molecular marker, includes the following steps:

[0064] (1) In July 2014, a hybrid combination was prepared at the Yuanyang Base of the Sesame Research Center of the Henan Academy of Agricultural Sciences using the sesame strain Yuzhi DS899 with high resistance to Fusarium wilt (DI 6.1%) and the germplasm with high susceptibility to Fusarium wilt JS012 (DI 100%) , get F 1 .

[0065] In November 2014, F was planted in the Sanya base 1 Seeds of the first generation, self-crossing of a single plant, and then obtaining F 2 Generation seeds, the population is more than 200 plants.

[0066] In February 2015, randomly selected 50 F 2 For the plump seeds of a single plant, F 2 Iden...

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Abstract

The invention belongs to the technical field of molecular genetic breeding, and in particular relates to a SNP molecular marker SiFWR2145 closely linked with a sesame resistance gene against Fusarium wilt. The position of the marker in the sesame SNP genetic map is 111.5~112.7cM, located in the 8th linkage group, and the interpretation rate of resistance to Fusarium wilt is 51.66% (Vg / Vp). The marker contains 107bp, and its sequence is shown in SEQ ID No .1 shown. Compared with the existing sesame breeding methods, the main advantages of the present invention are as follows: (1) The present invention is innovative for the sesame breeding technology, and compared with the currently applied conventional breeding technology methods, the detection method is simple and efficient, and the results are reliable; (2) the present invention The molecular marker SiFWR2145 provided by the Invention Institute can significantly improve the breeding efficiency when used in molecular assisted breeding, save scientific research costs and time, and lay a solid foundation for improving the sesame molecular breeding technology platform.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic breeding, and in particular relates to a SNP molecular marker closely linked with a sesame resistance gene against fusarium wilt SiFWR2145 . Background technique [0002] Sesame( Sesamum indicum L., 2n=26), belonging to the genus Linaceae, is an important characteristic high-quality oil crop in my country. my country is one of the four major producers of sesame, with an annual planting area of ​​about 12 million mu and a total output of about 750,000 tons. It plays an important role in the international oil trade. [0003] Sesame Fusarium wilt is one of the two main fungal diseases of sesame, which occurs widely in the world. Sesame Fusarium wilt caused by Fusarium oxysporum Sesame-specific ( Fusarium oxysporum f. sp. Sesami ) infection, mainly in the seedling and adult plant stages of sesame. The disease mainly occurs in Northeast my country, North China, Northwest China, Huan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 苗红梅张海洋魏利斌李春段迎辉常淑娴
Owner HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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