Method for detecting pesticides residues in tea or tea leaves

A technology for pesticide residues and detection methods, applied in the field of analytical chemistry, can solve problems such as fast and simple, and achieve the effects of simple detection methods, reduced use, safe and environmentally friendly dosage

Active Publication Date: 2015-12-23
MEASURING & TESTING TECHN RES INST FUJIAN PROV
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Therefore, in practical applications, it c

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  • Method for detecting pesticides residues in tea or tea leaves

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Embodiment 1

[0057] A method for detecting pesticide residues in tea or green tea, the detection steps of organophosphorus and carbamate pesticide residues in tea or green tea:

[0058] (1) Preparation of reagents:

[0059] Et reagent: Dissolve 2mg (or 1ku) cholinesterase in 500ml phosphate buffer solution (weigh 5~15g disodium hydrogen phosphate and 0.2~0.9g potassium dihydrogen phosphate, add 500mL distilled water to dissolve in the reagent bottle, mix well; ), add 2-10g of bovine serum albumin and 3-15g of trehalose and stir to dissolve; add 80-200mg of DTNB and 0.5-5g of sodium taurocholate and stir until completely dissolved to become Et reagent , encapsulated in 5ml ampoules and stored in a refrigerator (0-5°C). Just take it out and open it when you use it.

[0060] Sub reagent: add 30 mg of thioacetylcholine iodide to 10 mL of distilled water, shake well to dissolve;

[0061] Pesticide extract: add 200mL glycerol to 50mL phosphate or carbonate buffer solution, mix well, then add ...

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Abstract

The invention discloses a method for detecting pesticides residues in tea or tea leaves. A pesticide extraction reagent specially used for extracting residual pesticides in the tea leaves is developed against the special interference of endogenous substances in a tea or tea leaf sample to test, the pesticide residues in the tea leaves are extracted by using the reagent without adjustment of the pH value, chromatography column passing or an oxidation process, and the reagent has the advantages of safety, environmental protection and small use amount. Cholinesterase is initiatively compounded with DTNB to form an Et reagent, and the Et reagent and a sample solution to be detected directly undergo an enzyme inhibition reaction in the detection process to make the quantity of reagents added in the operation process be 1 lower than the variety quantity of reagents added in traditional operation processes, so the detection method is simple and reduces use of experiment apparatuses. The method is simple and fast, allows the full flow from sample processing to detection result reporting to be completed in 20min, and realizes multichannel simultaneous detection.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for detecting pesticide residues in tea or green tea. Background technique [0002] At present, there are the following two rapid detection methods for pesticides in tea that can be consulted, but both of them have the problems of cumbersome detection steps, time-consuming, and toxic and harmful reagents, so they cannot be popularized and applied in grass-roots laboratories and planting, production, and sales sites . The following is a brief introduction to the operation steps of the two methods: [0003] 1. In 2002, a recommended national standard "Easy Test Method for Organophosphorus and Carbamate Pesticide Residues in Tea Enzyme Inhibition Method" GB / T18625-2002 was issued, but because tea samples would cause special interference to the test, It is still unavoidable in the analysis method to be tedious and complicated, and it consumes a lot of pre-tr...

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Application Information

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IPC IPC(8): G01N1/28G01N21/01G01N30/02
Inventor 黄敏蔡琪刘薇张青松
Owner MEASURING & TESTING TECHN RES INST FUJIAN PROV
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