A kind of preparation method of high-calcium low-viscosity soybean protein
A soybean protein, low viscosity technology, applied in the direction of plant protein processing, etc., can solve the problems of limited ability to reduce viscosity, complex enzymatic hydrolysis conditions, difficult to control, etc., to achieve the effect of reducing viscosity, simple process and low cost
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[0011] A method for preparing high-calcium and low-viscosity soybean protein, the method comprising the following steps: (1) drying and crushing the low-temperature soybean meal, passing it through an 80-mesh sieve, and then dissolving it in an alkali solution to form a mixed solution. The alkali solution is 1mol / L NaOH and 2mol / L Ca(OH) 2 The mixed solution, the mass ratio of alkali solution to soybean meal is 7:1, the mixed solution is ultrasonically assisted to obtain soybean meal slurry, the ultrasonic power is 100-300W, the ultrasonic temperature is 50-70°C, and the ultrasonic time is 20-60min (2) Carry out high-pressure micro-jet homogenization treatment to the soybean meal slurry, the micro-jet homogenization pressure is 60-100MPa, the micro-jet homogenization time is 1-5min, then centrifuge to obtain the supernatant, adjust the supernatant The pH is 4.5 for acid precipitation, and then centrifuged to obtain a precipitate; (3) adding water to redissolve the precipitate,...
Embodiment 1
[0015] Dry and crush the low-temperature soybean meal and pass it through an 80-mesh sieve, and dissolve it in 1mol / L NaOH and 2mol / L Ca(OH) at a liquid-to-solid ratio of 7:1 2 In the mixed alkali solution, conduct ultrasonic-assisted extraction for 35 minutes under the condition of ultrasonic power of 200W and ultrasonic temperature of 65°C, then conduct high-pressure micro-jet homogenization for 4 minutes under the micro-jet homogenization pressure of 80 MPa, and centrifuge to obtain the supernatant solution, adjust the pH of the supernatant to 4.5 for acid precipitation, and then centrifuge to obtain a precipitate, redissolve the precipitate in water, adjust the pH to neutral, spray dry, and spray phospholipid oil to obtain high-calcium and low-viscosity soybean protein. The prepared soybean protein has low viscosity and calcium ion content greater than 5mg / g.
Embodiment 2
[0017] Dry and crush the low-temperature soybean meal and pass it through an 80-mesh sieve, and dissolve it in 1mol / L NaOH and 2mol / L Ca(OH) at a liquid-to-solid ratio of 7:1 2 In the mixed alkali solution, conduct ultrasonic-assisted extraction for 30 minutes under the condition of ultrasonic power of 250W and ultrasonic temperature of 60°C, then conduct high-pressure micro-jet homogenization for 5 min at a micro-jet homogenization pressure of 70 MPa, and centrifuge to obtain the supernatant solution, adjust the pH of the supernatant to 4.5 for acid precipitation, and then centrifuge to obtain a precipitate, redissolve the precipitate in water, adjust the pH to neutral, spray dry, and spray phospholipid oil to obtain high-calcium and low-viscosity soybean protein. The prepared soybean protein has low viscosity and calcium ion content greater than 5mg / g.
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