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PH responsive microcapsule type protein vaccine vector and preparation method thereof

A vaccine carrier and protein technology, which is applied in the fields of pharmaceutical formulations, allergic diseases, antibody medical components, etc., can solve problems that have not been reported, and achieve the effect of simple and easy preparation process, mild conditions, and cheap and easy-to-obtain raw materials

Inactive Publication Date: 2016-01-06
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation of microencapsulated protein vaccine carriers based on the coordination of Al(III) and tannic acid

Method used

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  • PH responsive microcapsule type protein vaccine vector and preparation method thereof
  • PH responsive microcapsule type protein vaccine vector and preparation method thereof
  • PH responsive microcapsule type protein vaccine vector and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a pH-responsive microcapsule protein vaccine carrier, comprising the steps of:

[0023] (1) 10mL, 0.33M sodium carbonate aqueous solution are poured in the calcium chloride aqueous solution that equal volume contains polystyrene sodium sulfonate, the concentration of described polystyrene sodium sulfonate is 2mg / mL, and the concentration of calcium chloride is 0.33M, stirred for 30s, centrifuged, washed 3 times with deionized water to obtain calcium carbonate particles;

[0024] (2) Disperse the calcium carbonate particles obtained in step (1) in 20 mL of deionized water, add 1 mL, 24 mM aqueous solution of tannic acid and vibrate on a vortex for 10 seconds to disperse evenly, add the same volume and concentration of the aqueous solution of tannic acid aluminum chloride aqueous solution, and vibrated on the vortex for 10s to disperse evenly, add 20mL, pH = 8.0 3-(N-morpholine) propanesulfonic acid sodium salt buffer solution to adjust the pH to 8....

Embodiment 2

[0027] A method for preparing a pH-responsive microcapsule protein vaccine carrier, comprising the steps of:

[0028] (1) Add protein mimetic antigen-chicken ovalbumin to 10mL, 0.33M sodium carbonate aqueous solution, make the concentration of chicken ovalbumin 2mg / mL, pour into equal volume chlorine containing sodium polystyrene sulfonate after dissolving In the calcium chloride aqueous solution, the concentration of sodium polystyrene sulfonate is 2 mg / mL, the concentration of calcium chloride is 0.33 M, stirred for 30 s, centrifuged, and washed with deionized water 3 times to obtain calcium carbonate particles containing mock antigens;

[0029] (2) Disperse the calcium carbonate particles containing the mock antigen obtained in step (1) in 20 mL of deionized water, add 1 mL of 24 mM tannic acid aqueous solution, oscillate for 10 seconds to disperse evenly, add the same volume and concentration of the tannic acid aqueous solution aluminum chloride aqueous solution, and vibra...

Embodiment 3

[0031] A method for preparing a pH-responsive microcapsule protein vaccine carrier, comprising the steps of:

[0032] (1) Add protein mimetic antigen-chicken ovalbumin into 10mL, 0.2M sodium carbonate aqueous solution to make the concentration of chicken ovalbumin 0.5mg / mL, dissolve and pour into an equal volume of sodium polystyrene sulfonate solution In the calcium chloride aqueous solution, the concentration of the sodium polystyrene sulfonate is 1 mg / mL, the concentration of the calcium chloride is 0.2 M, stirred for 20 s, centrifuged, and washed twice with deionized water to obtain calcium carbonate particles containing mock antigens;

[0033] (2) Disperse the calcium carbonate particles containing the mock antigen obtained in step (1) in 20mL deionized water, add 0.5mL, 12mM aqueous tannic acid solution, shake for 10s to disperse evenly, add the same volume as the aqueous tannic acid solution, etc. concentration of aluminum chloride aqueous solution, and vortex for 10s t...

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Abstract

The invention discloses a pH responsive microcapsule type protein vaccine vector and a preparation method thereof. The preparation method comprises the following steps: (1) adding a protein mimic antigen into a sodium carbonate aqueous solution, pouring into an isovolumetric calcium chloride aqueous solution containing sodium polystyrene sulfonate after dissolving, stirring, centrifuging, and washing with deionized water to obtain calcium carbonate particles containing the mimic antigen; and (2) dispersing the calcium carbonate particles containing the mimic antigen into deionized water, adding a tannic acid aqueous solution, oscillating to disperse uniformly, adding an aluminum chloride aqueous solution, oscillating to disperse uniformly, adding a 3-(N-morpholine) sulfonic acid sodium salt buffer solution to adjust the pH value, oscillating, centrifuging, washing the particles with deionized water, washing with EDTA (ethylene diamine tetraacetic acid) aqueous solution to remove calcium carbonate, and washing with deionized water to obtain the pH responsive microcapsule type protein vaccine vector. The preparation process of the pH responsive microcapsule type protein vaccine vector disclosed by the invention is implemented under a room-temperature water phase and the condition is mild, so that the activity of a protein vaccine and the use safety of the vector are ensured; and the raw materials are low in price and easy to obtain, and the preparation process is simple.

Description

technical field [0001] The invention relates to a pH-responsive microcapsule protein vaccine carrier and a preparation method, and belongs to the field of preparation of nano-micro materials. Background technique [0002] Traditional vaccines are inactivated or low-activity pathogens, which pose safety hazards during use. New vaccines (such as DNA, protein vaccines, etc.) are safe and effective, but they are easily degraded by enzymes and have weak immunogenicity. Other means are needed to protect the vaccine and stimulate the immune response. Traditional vaccine adjuvants are ineffective against these antigens, so the development of new vaccine vectors is urgently needed. [0003] Aluminum salts were the first adjuvants approved for use in humans. Aluminum salt adjuvant (Al(OH) 3 ) can effectively stimulate the humoral immune response mediated by B cells, but its specific mechanism of action is still unclear. It is generally considered to be the slow-release effect of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K39/39A61P37/04A61K47/64
Inventor 王晓莉孙洪范张超缪丹丹
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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