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Production method for powder containing crystalline alpha, alpha-trehalose dihydrate

A technology of dihydrate and crystalline powder, applied in the preparation of sugar derivatives, biochemical equipment and methods, sugar derivatives, etc., can solve problems such as inability to obtain, and achieve easy cultivation, high crystallinity, and excellent starch yield. Effect

Inactive Publication Date: 2016-01-06
HAYASHIBARA BIOCHEMICAL LAB INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is an inconvenience that in the case of the whole sugar method, the crystallized trehalose and the common impurities peculiar to such a production method of glucose contained in the massecuite are all pulverized together, and therefore, the obtained trehalose-containing The trehalose content in the powder of dihydrate crystals is not higher than the trehalose content of massecuite, and the powder containing high-purity trehalose dihydrate crystals cannot be obtained

Method used

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  • Production method for powder containing crystalline alpha, alpha-trehalose dihydrate
  • Production method for powder containing crystalline alpha, alpha-trehalose dihydrate
  • Production method for powder containing crystalline alpha, alpha-trehalose dihydrate

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Effect test

Embodiment 1

[0278]

[0279] Corn starch was suspended in water so as to be 30%, and calcium carbonate was added to this suspension to have a final concentration of 0.1%, and the pH was adjusted to 6.0. To this suspension, add 0.2% based on the mass of starch of heat-resistant α-amylase (trade name "Tamamil 60L", sold by Nobozaimzu Japan Co., Ltd.), and react at 98 to 100° C. for 15 minutes. Starch gelatinization - liquefaction. After the obtained liquefied starch solution was treated in an autoclave at 125°C for 15 minutes, it was cooled to 51°C. To this solution, 2 units and 10 units of α- Partially refined enzyme solution of glycosyl trehalose synthase and trehalose separation enzyme, in addition, 300 units of isoamylase (manufactured by Hayashibara Co., Ltd.) per 1 gram of starch, and 2 units of isoamylase derived from experiments 1-2 The prepared CGTase of Paenibacillus illinois NBRC15959 strain was further reacted for about 70 hours. Next, this reaction solution was heated at 97°...

Embodiment 2

[0282]

[0283] Using 3 units and 15 units of α-glycosyl trehalose synthase and trehalose separation enzyme per 1 gram of starch respectively, the reaction time reaches 40 hours. Except for the CGTase of Paenibacillus NBRC15379 strain, trehalose production reaction and glucoamylase treatment were performed in the same manner as in Example 1, thereby obtaining the purity of trehalose, that is, the purity of trehalose in terms of anhydrous matter. A reaction solution with a trehalose content of 87.6%. Heating the obtained reaction solution to inactivate the enzyme, decolorize and filter the filtrate through activated carbon according to conventional methods, desalt the filtrate with cation exchange resin (H+ type) and anion exchange resin (OH- type), and concentrate under reduced pressure to form a solid concentration of about 85 % concentrate. Put it in an auxiliary crystallization tank, add 1% reagent-grade powder containing trehalose dihydrate crystals (trade name "trehalo...

Embodiment 3

[0287]

[0288]As the CGTase, except that the CGTase derived from the Paenibacillus NBRC13638 strain prepared by the method of Experiment 1-2 was used, in the same manner as in Example 1, the trehalose production reaction was performed, and then the glucoamylase The trehalose content of the reaction liquid after the treatment was 87.2% in terms of anhydrous. The reaction liquid thus obtained is heated to inactivate the enzyme, and decolorized and filtered through activated carbon according to a conventional method, and the filtrate is desalted with a cation exchange resin (H+ type) and an anion exchange resin (OH- type), and concentrated under reduced pressure to form a solid concentration About 85% concentrate. Put it in an auxiliary crystallization tank, add 1% reagent-grade powder containing trehalose dihydrate crystals (trade name "trehalose 999", code number: TH224, trehalose purity 99.9% or more, sold by Hayashibara Co., Ltd.) As a seed crystal, bring it to 60°C while...

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Abstract

The invention relates to a production method for a powder containing crystalline alpha, alpha-trehalose dehydrate, and in particular to a production method for a powder containing crystalline alpha, alpha-trehalose dehydrate and a powder containing crystalline alpha, alpha-trehalose dehydrate prepared by the production method. The production method prepares from starch by the consecutive steps, has high yield, and is suitable for industrial preparation of the powder containing crystalline alpha, alpha-trehalose dehydrate with high purity.

Description

[0001] This application is a divisional application of an invention patent application with an application date of September 12, 2012, an application number of 201280055581.8, and an invention title of "Preparation method of powder containing α,α-trehalose dihydrate crystals". 【Technical field】 [0002] The present invention relates to a method for preparing a powder containing α,α-trehalose dihydrate crystals, in particular, to a method for preparing a powder containing α,α-trehalose dihydrate crystals and the product obtained by the preparation method Powder containing α,α-trehalose dihydrate crystals, said method is suitable for industrially producing high-purity α,α-trehalose dihydrate crystals containing powders from starch through successive steps in excellent yield powder. 【Background technique】 [0003] As a method for producing a powder of dihydrate crystals containing α,α-trehalose (hereinafter, this specification is simply referred to as “trehalose”), various meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04
CPCC07H3/04C12P19/18C07H1/00C12P19/14C12N9/1074C12Y204/01019C12P19/12C12P19/20C13K13/007
Inventor 涩谷孝伊泽精祐
Owner HAYASHIBARA BIOCHEMICAL LAB INC
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