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Method for preparing zymosan, mycose and yeast extract from beer waste yeast

A technology for waste beer yeast and yeast extract, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of maximization, failure to realize resource utilization, and failure to fully realize comprehensive utilization of waste beer yeast. , to achieve the effect of increasing added value, promoting protein hydrolysis, and improving flavor

Inactive Publication Date: 2009-07-15
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcoming of these current technologies is that the comprehensive utilization of beer waste yeast has not been fully realized, so the maximum utilization of resources has not been realized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Fresh beer waste yeast passed through a 100-mesh sieve is continuously centrifuged at 3000g to obtain beer waste yeast with a water content of about 77.5-80% for use; add 100kg of water at 5°C to the mixing tank, and add 0.6kg of NaHCO 3 , stirred to dissolve completely, NaHCO 3 The solution was added to 50kg of beer waste yeast sludge, stirred for 30 minutes, and centrifuged continuously at 3000g to obtain a solid isolate for subsequent use;

[0028] Put the debittered yeast into the reaction tank, add 100kg of water and stir evenly, adjust the pH to 6.5 with hydrochloric acid, then add 0.65kg of salt, 0.65kg of potassium chloride, 15g of magnesium chloride and 1.5g of wall-breaking enzyme and stir evenly, at 45 Incubate enzymatic autolysis at ℃ for 24 hours;

[0029] Raise the temperature of the yeast liquid that has been preliminarily enzymatically autolyzed to 50°C, add 15g of yeast extract enzyme, further enzymatically hydrolyze for 24 hours, then raise the temper...

Embodiment 2

[0034] The fresh waste beer yeast passed through a 100-mesh sieve is continuously centrifuged at 3000g to obtain waste beer yeast sludge with a water content of about 77.5-80% for subsequent use;

[0035] Add 100kg 10℃ water to the mixing tank, add 1.2kg NaHCO 3 , stirred to make it completely dissolved, added 50kg of beer waste yeast mud, stirred for 10 minutes, and centrifuged continuously at 3000g to obtain a solid isolate for subsequent use;

[0036] Put the debittered yeast into the reaction tank, add 100kg of water and stir evenly, adjust the pH to 6.0 with acid, then add 1.3kg of salt, 1.3kg of potassium chloride, 0.65kg of magnesium chloride and 67.5g of wall-breaking enzyme and stir evenly. Incubate enzymatic autolysis at 50°C for 8 hours;

[0037] Raise the temperature of the yeast liquid that has been preliminarily enzymatically autolyzed to 55°C, add 200g of yeast extract enzyme, further enzymatically hydrolyze for 8 hours, then raise the temperature to 75°C, and ...

Embodiment 3

[0042]The fresh spent beer yeast passed through a 100-mesh sieve is continuously centrifuged at 3000g to obtain spent beer yeast with a water content of about 77.5-80% for subsequent use;

[0043] Add 100kg of 8℃ water to the mixing tank, add 0.9kg of NaHCO 3 , stirred to make it completely dissolved, added 50kg of beer waste yeast mud, stirred for 10 minutes, and centrifuged continuously at 3000g to obtain a solid isolate for subsequent use;

[0044] Put the debittered yeast into the reaction tank, add 100kg of water and stir evenly, adjust the pH to 7.0 with acid, then add 1.0kg of salt, 1.0kg of potassium chloride, 0.1kg of magnesium chloride and 150g of wall-breaking enzyme, and then stir evenly. Incubate enzymatic autolysis at ℃ for 6 hours;

[0045] Raise the temperature of the yeast liquid that has undergone preliminary enzymatic autolysis to 60°C, add 140g of yeast extract enzyme, further enzymatically hydrolyze for 12 hours, then raise the temperature to 85°C, and ke...

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PUM

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Abstract

The invention discloses a method of using waste bear yeast to simultaneously prepare zymosan, trehalose and yeast extract. The method comprises steps of debittering and impurities removing of the waste bear yeast, autolysis, enzymatic hydrolysis and inactivation of the waste bear yeast, separation and drying of the zymosan, membrane separation, concentration, crystallization and drying of the trehalose, concentration and drying of the yeast extract, etc. The method has the characteristics of high raw material utilization rate, good quality, low cost and easy industrialization, realizes that three products of the zymosan, the trehalose and the yeast extract are simultaneously produced from the same industrial waste, the waste bear yeast, improves the utilization rate of industrial wastes and achieves the target of maximal waste resource recovery.

Description

technical field [0001] The invention relates to a method for preparing zymosan, trehalose and yeast extract by using yeast, in particular to a method for preparing zymosan, trehalose and yeast extract by using beer waste yeast. Background technique [0002] Beer waste yeast is a by-product of beer production. According to the production of 100t beer, 1.5t of beer waste yeast with a moisture content of 75% to 80% can be obtained. At present, my country produces about 600,000 tons of beer waste yeast every year. If it is discharged with wastewater Not only wasting precious resources, but also causing serious environmental pollution. [0003] Beer waste yeast is rich in protein, trehalose and other nutrients. The supernatant after yeast autolysis can be made into yeast extract, which is a nutrient-rich and cheap protein source, and can be used to prepare excellent food seasonings with three functions of nutrition, seasoning and health care. [0004] The supernatant after yeast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/04C12P1/02C12R1/645
Inventor 朱明军梁世中方贵权田小群
Owner SOUTH CHINA UNIV OF TECH
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